氯氰菊酯手性拆分及其抗体立体选择性研究

doi:10.3969/j.issn.1673-1689.2013.12.009

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氯氰菊酯手性拆分及其抗体立体选择性研究
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                        10.3969/j.issn.1673-1689.2013.12.009
                    
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基金项目:国家“十二五”科技支撑计划项目(2012BAC01B07,2012BAD29B04);国家863计划项目(2012AA06A303)

Separation of Cypermethrin and Stereoselectivity of Antibodies to Cypermethrin Isomers

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以HP-silica柱和Sino-Chiral OD手性柱为固定相,通过紫外检测器检测,采用Waters484型液相色谱仪对氯氰菊酯进行手性拆分和单体制备;并根据Sino-Chiral OD手性柱的分离结果和文献,对氯氰菊酯异构体进行构型鉴定;最后,研究了抗氯氰菊酯单克隆抗体对其8个异构体的抑制效果。研究表明:抗氯氰菊酯单克隆抗体仅对氯氰菊酯的2个异构体(CP1,1R-cis-αR和CP2,1R-cis-αS)有抑制作用;抗体对CP1的灵敏度比对CP2要高得多,对高杀虫活性CP2异构体的半数抑制浓度为23.62 ng/mL,为将来高活性氯氰菊酯异构体的免疫检测提供了依据。

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With the developing of chromatographic separation,it is inevitable that cypermethrin isomers with high insecticidal activities would be prepared. In this work,HP-silica column and Sino-Chiral OD column were chosed as the stationary phase and examined using the ultraviolet detector to separation and isomer’s preparation of cypermethrin by the liquid performance spectrometer of Waters 484. Then,the configurations of cypermethrin isomers were identified according to the separation results by Sino-Chiral OD column and the reported studies. Finally,the inhibition effect of monoclonal antibodies to cypermethrin isomers was performed. The results demonstrated that only two of eight cypermethrin isomers,CP1(1R-cis-R) and CP2(1R-cis-S), were recognized by the antibodies,and CP1 was more sensitivity than CP2. Fifty percent inhibition concentration of CP2 with high insecticidal activities was 23.62 ng/mL. This results indicated that immunological technology for the determination of CP2 isomer could be established in the future.

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陈秀金,匡华,刘丽强,胥传来.氯氰菊酯手性拆分及其抗体立体选择性研究[J].食品与生物技术学报,2013,32(12):1287-1292.

CHEN Xiu-jin, KUANG Hua, LIU Li-qiang, XU Chuan-lai. Separation of Cypermethrin and Stereoselectivity of Antibodies to Cypermethrin Isomers[J]. Journal of Food Science and Biotechnology,2013,32(12):1287-1292.

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在线发布日期: 2014-06-17
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