Abstract:Biosynthesis of protein and amino acids by Chlorella vulgaris inoculated in different culture modes were studied,respectively. Chlorella strain was cultivated to synthesize protein in autotrophism,heterotrophism and mixotrophism models,respectively. The results were as follows:the mixotrophic algal cell could accumulate higher biomass,with a specific growth rate and productivity of 0.526 d-1and 0.318 mg/(L·d),respectively. Its high protein productivity was of 0.097 g/(L·d), but the protein content in autotrophic Chlorella was the highest(52.1%),which the algal protein contained 18 kinds of amino acids in the three models and the percentage of essential amino acids was 40.0% of the total;under heterotrophism and mixotrphism models,proportions of amino acids from the algal protein were rather similar,compared with the algae in autotrophism model,proportions of Cys-s,Tyr and Met were the significant difference,additionally Cys-s proportions in the previous two were about 78.57% higher than the later,while proportions of Tyr and Met were about 27.59% and 33.33% lower than the previous two,respectively. Consequently,biosynthesis of protein and proportion of the algal amino acids were both significantly influenced in the culture models.
