Abstract:The protective effects of fermentation broth of Lyophyllum ulmarium(LU) on human umbilical vein endothelial cells(HUVEC) were observed. The H2 O2 was used to induce the injury of HUVEC after the treatment with 20,40 and 80 mg/L LU. Survival rate of HUVECs was determined by the MTT assay,the lactate dehydrogenase(LDH) activity in culture serum was investigated using the colorimetric method,the apoptotic rate of HUVECs was detected by flow cytometry,and the protein expression of bcl-2,bax,Caspase-3 and Caspase-9 was detected by western blot method. The results showed that LU could increase the survival rate of endothelial cells induced by H2 O2 and increase LDH activity,could reduce the cell apoptostic rate induced by H2 O2,down-regulated the expression of bax,Caspase-3,Caspase-9 proteins,and up-regulated the expression of bcl-2 protein. It was indicated that the fermentation broth of Lyophyllum ulmarium has protective effects on vascular endothelial cell,probably via the inhibition of apoptosis.
