高效毛细管电泳法测定食品中5种甜味剂含量

doi:10.3969/j.issn.1673-1689.2014.03.014

首页 > 过刊浏览>2014年第33卷第3期 >315-320. DOI:10.3969/j.issn.1673-1689.2014.03.014

高效毛细管电泳法测定食品中5种甜味剂含量
DOI:
                        10.3969/j.issn.1673-1689.2014.03.014
                    
作者:
                        
                        
                    
作者单位:
作者简介:
通讯作者:
中图分类号:
基金项目:安徽省自然科学基金项目(1308085QB24);安徽省高校省级优秀青年人才基金重点项目(2013SQRL051ZD);安徽省生化药物工程技术研究中心开放课题(BYEC1202,BYEC1301)

Simultaneous Determination of 5 Sweeteners in Food by HPCE

Author:

Affiliation:

Fund Project:

摘要

图/表

访问统计

参考文献

相似文献

引证文献

资源附件

文章评论

摘要:

采用高效毛细管电泳-紫外可见检测法同时测定食品中5种人工合成甜味剂纽甜、阿斯巴甜、甜蜜素、糖精钠、安赛蜜的含量。内壁未涂层熔融石英毛细管(50μm×75 cm,有效长度60 cm),毛细管温度为25℃;缓冲液为0.2 mol/L硼酸盐(pH=8.29),分离电压+23 kV,进样压力3.45 kPa,进样时间3.0 s,UV-Vis检测器检测波长200 nm。结果显示:5种甜味剂线性回归方程相关系数r在0.999 21~0.999 89之间,定量限在2.2~552.6 mg/L之间,样品加标回收率在96.29%~102.31%范围内,相对标准偏差在3.81%~6.40%范围内。表明该方法简便、灵敏、快速、可靠、重现性好,能用于食品中5种甜味剂的同时测定。

Abstract:

The method of simultaneous determination of five kinds of artificial sweetener neotame, aspartame,sodium cyclamate,sodium saccharin,acesulfame content was established by HPCE with UV-Vis detector in this manuscript. The fused silica capillary with uncoated inner wall(50 μm×75 cm,effective length 60 cm) was used,and the capillary was kept at 25 ℃. 0.2 mol/L borate(pH= 8.29) was adopted as electrophoresis buffer at +23 kV separation voltage. The sample was injected under 3.45 kPa pressure for 3.0 seconds,and detection wavelength being set at 200 nm. The linear regression equation correlation coefficient r for five kinds of sweeteners was between 0.999 21 and 0.999 89. The detection limit of five kinds of sweeteners was in the range of 2.2~552.6 mg/L. The spiked recoveries were within the range from 96.29% to 102.31%,and the relative standard deviation was kept between 3.81% and 6.40%. Conclusion The method is simple,sensitive,rapid, reliable and well reproducible,it can be used for the determination of five sweeteners in food simultaneously.

参考文献

相似文献

引证文献

引用本文

薛洪宝,张晖,梁丽丽,杨俊松,申林,陶兆林.高效毛细管电泳法测定食品中5种甜味剂含量[J].食品与生物技术学报,2014,33(3):315-320.

XUE Hongbao, ZHANG Hui, LIANG Lili, YANG Junsong, SHEN Lin, TAO Zhaolin. Simultaneous Determination of 5 Sweeteners in Food by HPCE[J]. Journal of Food Science and Biotechnology,2014,33(3):315-320.

复制

文章指标

点击次数:
下载次数:
HTML阅读次数:
引用次数:

历史

收稿日期:
最后修改日期:
录用日期:
在线发布日期: 2014-06-16
出版日期:

地址：江苏省无锡市蠡湖大道1800号邮政编码：214122

电话：0510-85913526 电子邮件：xbbjb@jiangnan.edu.cn

技术支持：北京勤云科技发展有限公司

引用本文

分享

文章指标

历史

微信公众号二维码

手机版网站二维码