基于形态特征和ITS序列分析秦巴山区黑木耳主要栽培种的亲缘关系

doi:10.3969/j.issn.1673-1689.2014.05.014

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基于形态特征和ITS序列分析秦巴山区黑木耳主要栽培种的亲缘关系
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                        10.3969/j.issn.1673-1689.2014.05.014
                    
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Analysis of the Genetic Relationships of Main Auricularia auricular Cultispecies in Qinba Mountainous Area Based on ITS Sequence

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采用CTAB法提取黑木耳菌株的DNA，进行PCR扩增，扩增产物进行测序。耳2和标准菌株Auricularia auricula-judae HF-8（GQ168492）聚类在同一分支上；耳913和标准菌株Auricularia auricula-judae YBS-7（GQ168503）聚类在同一分支上，且神农A8与新科、耳2、耳913亲缘关系相对较远。耳97-2和标准菌株Auricularia auricula-judae NW482（EU520194）聚类在同一分支上；耳268和标准菌株Auricularia auricula-judae NW491（EU520160）聚类在同一分支上，且秦单4#与耳97-2、耳268亲缘关系相对较远。新科、耳2、耳913、神农A8此4个菌种亲缘关系比较近；耳268、秦单4#、耳97-2等3个菌种亲缘关系较近。通过黑木耳菌株的形态观察及ITS序列分析，得到两株亲缘关系较远的优势菌株秦单4#和神农A8。

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Analyzing the genetic relationships of the main Auricularia auricular cultispecies in Qinba mountainous area by ITS sequence so as to lay a theoretical foundation to genetics and breeding. Using CTAB method to extract genomic DNA from the bacterial strain, Auricularia auricular, and amplifying and sequencing PCR products; Auricularia auricula-judae 2 and Auricularia auricula-judae HF-8(GQ168492) were clustered in the same branch, Auricularia auricula-judae 913 and Auricularia auricula-judae YBS-7(GQ168503) were clustered in the same branch, but the genetic relationship between Auricularia auricula-judae Shennong A8 and the Auricularia auricula-judae 2, Auricularia auricula-judae 913 were relatively alienative. Auricularia auricula-judae 97-2, Auricularia auricula-judae NW482(EU520194), the Auricularia auricula-judae 268 and Auricularia auricula-judae NW491 (EU520160) were clustered in the same branch. However the genetic relationships among Auricularia auricula-judae Qindan 4, Auricularia auricula-judae 97-2, Auricularia auricula-judae 268 were relatively alienative. The genetic relationships among Auricularia auricula-judae Xinke, Auricularia auricula-judae 2, Auricularia auricula-judae 913 and Auricularia auricula-judae Shennong A8 were close. However, the relationships between Auricularia auricula-judae 268, Auricularia auricula-judae Qindan 4# and Auricularia auricula-judae 97-2 were quite far. Through the morphologic and ITS analysis of Auricularia auricular strains, two dominant strains, Auricularia auricula-judae Qindan 4# and Auricularia auricula-judae Shennong A8, have alienative relationship. Our findings established a solid foundation to genetics and breeding.

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王进,陈文强,邓百万,彭浩,解修超,赵娜娜,刘兆迪,乔艳明,张红涛.基于形态特征和ITS序列分析秦巴山区黑木耳主要栽培种的亲缘关系[J].食品与生物技术学报,2014,33(5):535-541.

WANG Jin, CHEN Wenqiang, DENG Baiwan, PENG Hao, XIE Xiuchao, ZHAO Nana, LIU Zhaodi, QIAO Yanming, ZHANG Hongtao. Analysis of the Genetic Relationships of Main Auricularia auricular Cultispecies in Qinba Mountainous Area Based on ITS Sequence[J]. Journal of Food Science and Biotechnology,2014,33(5):535-541.

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在线发布日期: 2014-10-19
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