Abstract:Effective components were extracted from deer blood by ethanol in this study, firstly separated through the MCI column, and then purified by ODS reversed phase chromatographic column, repeated separation. Finally, three kinds of compounds were extracted., their structures were identified by 1HNMR, 13CNMR et al: the compound Ⅰwas △4, 14-androstene-2 alkanol-28, 30 Cyclopentadiene heptanoic acid ester-3 ketone; the compound Ⅱwas △4, 14-androstene-2, 19 alkanol-28, 30 Cyclopentadiene heptanoic acid ester-3 ketone; the compound Ⅲ was △4, 14-androstene-2 alkanol-27, 29 Cyclopentadiene hexyl ether -3 ketone.The results shown that three compounds are all male hormone derivatives, they are all firstly reported in deer blood.
