Abstract:To investigate the protective effects of resveratrol against oxidative damage induced by high glucose for different time in HepG2 cell. The cells were treated with 33 mmol/L glucose to induce oxidative damage and with different concentrations of Res 0.1, 1, 10 μmol/L for 24, 48, and 72 h. The cell viability was measured by MTT assay, DCFH-DA was used as fluorescence probe for observing ROS level in cells, superoxide dismutase(SOD), malondialdehyde(MDA), glutathione peroxidase(GSH-PX), total antioxidative capacity(T-AOC) were determined by using commercially available kits. Real-time PCR was used to determine the relative mRNA expression of nuclear factor-E2-related factor(Nrf2) and hemeoxygenase-1(HO-1). Results show that compared with high glucose damage group, after 48h, cell viability of Res groups was significantly increased(p<0.05), ROS level was significantly decreased(p<0.05). In Res groups, cell redox state was improved, 10 μmol/L Res group was significantly improved for 48 h(p<0.01), its Nrf2 HO-1 mRNA levels was significantly up-regulated(p<0.01). It is a significant protective effect of resveratrol against oxidative damage induced by high glucose in HepG2 and it works through scavenging free radicals, improving cell redox state and up-regulating Nrf2 HO-1 mRNA levels.
