Abstract:A ultra performace liquid chromatography(UPLC) method was applied to determinating the contents of betulin,ergosterol,lanosterol,cholesterol,stigmasterol and sitosterol in the submerged-cultured mycelia of Inonotus obliquus. The procedure was carried out on a shim-pack XR-ODSⅢ column(150 mm×2 mm,2.2 μm),using acetonitrile(100%) as mobile phase with a flow rate of 0.8 mL/min. The detecting wavelength was 202 nm and the column temperature was 30 ℃. This method had good reproducibility and satisfactory recoveries The relative standard deviations of this method were less than 0.10%~0.33%(n=5) and 0.07%~1.66%(n=5) for intraday and inter-day assays,respectively. A good linear correlation was obtained in a range of 0.3~1.6 μg. The recoveries of betulin,ergosterol,lanosterol,cholesterol,stigmasterol,and sitosterol were 97.05%,98.74%,95.65%,101.45%,96.62%和96.14%,respectively. This method could be applied to evaluate real samples,and it was rapid,accurate and suitable for the quantitative determination of the six steroids in the submerged-cultured mycelia of Inonotus obliquus.
