培养条件对Streptococcus equisimilis合成透明质酸相对分子质量的影响

doi:10.3969/j.issn.1673-1689.2015.02.016

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培养条件对Streptococcus equisimilis合成透明质酸相对分子质量的影响
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                        10.3969/j.issn.1673-1689.2015.02.016
                    
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Effect of Culture Condition on the Molecular Weight of Hyaluronic Acid Synthesized by Streptococcus equisimilis

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作者比较了不同培养条件对Streptococcus equisimilis合成透明质酸相对分子质量的影响。结果表明:高质量浓度葡萄糖有利于长链透明质酸的合成,当发酵体系葡萄糖初始质量浓度从20 g/L上升至80 g/L,相对分子质量从1.24×106增大到2.02×106,提高了62.9%。葡萄糖补料培养不利于高相对分子质量透明质酸的合成,利用葡萄糖间歇和连续补料培养的方式得到透明质酸相对分子质量分别为1.53×106和1.42×106,比分批培养下降了19.9%和25.7%。在33~39 ℃范围内,较低温度有利于高相对分子质量透明质酸的合成,在33 ℃培养条件下透明质酸相对分子质量最高可达2.54×106。发酵液pH显著影响透明质酸的相对分子质量,在pH 8时,达到了最高的2.38×106。较高溶氧水平有利于高相对分子质量透明质酸的合成,在0~45%溶氧浓度范围内,相对分子质量随溶氧水平的增加从1.16×106提升至2.43×106,增长了109.4%。本研究结果为后续高相对分子质量透明质酸的生产提供有用的实验依据。

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The molecular weight of hyaluronic acid significantly affected its physicochemical properties and application effects. Effects of different culture conditions on the molecular weight of hyaluronic acid synthesized by Streptococcus equisimilis was compared,the results of which indicated that glucose with high concentration benefited the synthesis of long-chain hyaluronic acid. The concentration of glucose had significantly effect on the molecular weight of hyaluronic acid. With the initial concentration of glucose enhancing from 20 g/L to 80 g/L,the molecular weight of hyaluronic acid was increased to 2.02×106 from 1.24×106,i.e.,increased by 62.9%. Feeding mode of glucose significantly affected the molecular weight of hyaluronic acid. The molecular weight of hyaluronic acid was respectively 1.53×106 and 1.42×106 through interval and continuous feeding modes of glucose,which was decreased by 19.9% and 25.7% by comparing with batch culture. Low temperature was beneficial to the synthesis of high-molecular-weight hyaluronic acid. Under the culture condition of 33 ℃,the molecular weight of hyaluronic acid could reach to 2.54×106 at the maximum. pH also significantly affected the molecular weight of hyaluronic acid. When pH was 8,the molecular weight of hyaluronic acid reached to the highest 2.38×106. Dissolved oxygen with higher level benefited the sythesis of high-molecular weight hyaluronic acid,within the concentration range of 0~45% of dissolved oxygen,the molecular weight was increased to 2.43×106 from 1.16×106 with the increasing concentration of dissovled oxygen,increased by 109.4%.

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刘金龙,赵国群,李志敏,高敏杰,.培养条件对Streptococcus equisimilis合成透明质酸相对分子质量的影响[J].食品与生物技术学报,2015,34(2):209-214.

LIU Jinlong, ZHAO Guoqun, LI Zhimin, GAO Minjie,. Effect of Culture Condition on the Molecular Weight of Hyaluronic Acid Synthesized by Streptococcus equisimilis[J]. Journal of Food Science and Biotechnology,2015,34(2):209-214.

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