西瓜花叶病毒三抗体夹心法与纸质免疫检测传感器检测方法的建立
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

基金项目:


TAS-ELISA and Electrochemical Paper Assisted Immunosensor for the Detection of Watermelon Mosaic Virus
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    利用西瓜花叶病毒2号外壳蛋白的小鼠单克隆抗体与兔多克隆抗体,建立两种针对西瓜花叶病毒2号的检测方法。运用现有的抗体建立了三抗体夹心酶联免疫吸附法(TAS-ELISA);运用碳纳米管与抗体包裹滤纸制备纸质免疫检测传感器,再基于电化学工作站建立电化学纸质免疫检测传感器检测方法。两种方法对实际样品检测限分别为0.15、0.20 μg/mL,检测时间分别为3 h和30 min。根据结果分析比较,三抗体夹心法具有较高的检测灵敏度,而电化学纸质免疫传感器检测方法具有更短的检测时间。

    Abstract:

    Using a mice monoclonal antibody and a rabbit polyclonal antibody of the watermelon mosaic virus (WMV)-2 coat protein, we developed two detection methods for WMV-2. The trimer antibody sandwich enzyme-linked immunosorbent assay (TAS-ELISA) method was established based on two different antibodies; the electrochemical paper assisted immunosensor detection method was established by combining the electrochemical workstation with the paper assisted immunosensor prepared by the carbon nanotube and the antibody-coated filter paper. The detection limit was 0.15 and 0.2 μg/ml, and the testing time was 3 hours and 30 minutes for two methods, respectively. According to these results, the TAS-ELISA method had a higher detection sensitivity, and the electrochemical paper assisted immunosensor detection method had a shorter testing time.

    参考文献
    相似文献
    引证文献
引用本文

孔德昭,朱怡橙,马伟,徐丽广,胥传来.西瓜花叶病毒三抗体夹心法与纸质免疫检测传感器检测方法的建立[J].食品与生物技术学报,2015,34(4):367-371.

KONG Dezhao, ZHU Yicheng, MA Wei, XU Liguang, XU Chuanlai. TAS-ELISA and Electrochemical Paper Assisted Immunosensor for the Detection of Watermelon Mosaic Virus[J]. Journal of Food Science and Biotechnology,2015,34(4):367-371.

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2015-08-09
  • 出版日期:

版权所有:《食品与生物技术学报》编辑部

地址:江苏省无锡市蠡湖大道1800号  邮政编码:214122

电话:0510-85913526  电子邮件:xbbjb@jiangnan.edu.cn

技术支持:北京勤云科技发展有限公司

微信公众号二维码

手机版网站二维码