基于SPR技术的磺胺二甲氧嘧啶高灵敏检测

doi:10.3969/j.issn.1673-1689.2015.07.005

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基于SPR技术的磺胺二甲氧嘧啶高灵敏检测
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                        10.3969/j.issn.1673-1689.2015.07.005
                    
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High Sensitive Detection of Sulfadimethoxine Based on SPR Technique

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摘要:

磺胺类药物的过量使用会对人体造成过敏性反应，并可能具有致癌性，而磺胺二甲氧嘧啶(Sulfadimethoxine，SDM)在食品中磺胺类药物的使用中占有很大的比例，因此基于表面等离子体共振(Surface plasmon resonance，SPR)技术建立了奶粉样品中SDM的快速、高灵敏检测方法。通过待测样品与SPR芯片表面修饰的SDM抗体分子特异性识别引起金膜表面折射率变化，进而导致SPR角的变化以实现检测。建立SPR响应信号和SDM浓度间的标准曲线，最低检测限达到0.19 ng/mL，奶粉样品添加回收率在94.0%～98.3%。和传统方法相比，SPR技术具有较高的灵敏度、准确性且操作简便，SPR传感芯片完整性好且可重复用于实际样品检测中。

Abstract:

The overdose of sulfa drugs will cause allergic reactions and possibly carcinogenic. Among the all sulfa drugs used in food，sulfadimethoxine(SDM) takes up a large proportion. In this research，a new method based on surface plasmon resonance(SPR) was proposed for the rapid and high sensitive detection of SDM in milk powder. The detection was based on the changes of SPR angles，which was subsequently caused by the changes in the refractive index of gold film induced by the specific recognition of samples by the SDM antibody modified on the surface of SRP chips. A standard curve represented the relationship between SRP response signal and SDM concentration. The limit of detection(LOD) for SDM was as low as 0.19 ng/mL，and the milk powder recovery was in the range of 94.0%～98.3%. Compared with the traditional methods，SPR technique has high sensitivity，high accuracy and easy operation. SPR sensor chips can be repeatedly used for sample detection in practical application with good integrity.

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赵媛,徐丽广,吴晓玲,刘丽强,胥传来.基于SPR技术的磺胺二甲氧嘧啶高灵敏检测[J].食品与生物技术学报,2015,34(7):699-703.

ZHAO Yuan, XU Liguang, WU Xiaoling, LIU Liqiang, XU Chuanlai. High Sensitive Detection of Sulfadimethoxine Based on SPR Technique[J]. Journal of Food Science and Biotechnology,2015,34(7):699-703.

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在线发布日期: 2015-11-28
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