9顺维A酸对人肺腺癌细胞A2增殖及凋亡影响的实验研究

doi:10.3969/j.issn.1673-1689.2015.07.017

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9顺维A酸对人肺腺癌细胞A2增殖及凋亡影响的实验研究
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                        10.3969/j.issn.1673-1689.2015.07.017
                    
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Effects of 9-Cis-Retinoic Acid on the Proliferation and Apoptosis of Human Lung Cancer Cell Line A2

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研究9顺维A酸(9-cis RA)抑制肺腺癌细胞A2增殖及诱导凋亡的作用并初步探讨其作用机制。体外培养A2细胞，随机分为4组，实验组加9-cis RA 使其终浓度为1、5、10 μmol/L，对照组加入二甲亚砜使其终质量分数为0.1%，采用MTT法检测各组A2细胞增殖情况；使用流式细胞术(flow cytometry，FCM)分析药物作用后各组细胞的凋亡率；用逆转录-聚合酶链反应(RT-PCR)检测各组细胞bax、fas、bcl-2基因的变化。9顺维A酸对人肺癌细胞株A2增殖均有明显的抑制作用(P<0.01)，实验组中细胞凋亡发生率显著增高(P<0.05)，且随着浓度的升高抑制作用和凋亡率也随之提高。实验组bax mRNA、fas mRNA表达显著高于对照组，差异有统计学意义(P<0.01)；实验组bcl-2 mRNA表达显著低于对照组，差异具有统计学意义(P<0.01)。结论 9-cis RA具有明显的抗肺腺癌细胞A2增殖的作用，其机制可能通过上调bax、fas基因和下调bcl-2基因表达、诱导细胞凋亡有关。

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The study aimed to explore the mechanism of 9-cis-retinoic acid(9-cis RA) inhibiting proliferation and inducing apoptosis in human lung cancer cell line A2.Cultured A2 cells were randomly divided into four groups，the experimental group received 9-cis RA to a final concentration of 1，5 or 10 μmol/L，and the control group was added dimethyl sulfoxide to a final concentration of 0.1%. The MTT method was used for the determination of cell proliferation，flow cytometry(FCM) for the analysis of apoptosis，and reverse transcription-polymerase chain reaction(RT-PCR) for the expression changes of bax，fas and bcl-2 genes. Results showed that 9-cis RA significantly inhibited the cell proliferation(P<0.01) and the incidences of apoptosis in the experimental groups were significantly higher(P<0.05)，which depended on the concentration of 9-cis RA.The mRNA expressions of bax and fas genes in the experimental groups were significantly higher(P<0.01) whereas the bcl-2mRNA expressionwas significantly lower than the control group(P<0.01).Therefore，9-cis RA significantly inhibitsthe proliferation of human lung cancer cell line A2，which may be related to the increased expressions of bax and fas genes and the decreased expression of bcl-2 gene，and then induced apoptosis.

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酆渊,茆勇,游庆军,徐静静.9顺维A酸对人肺腺癌细胞A2增殖及凋亡影响的实验研究[J].食品与生物技术学报,2015,34(7):779-784.

FENG Yuan, MAO Yong, YOU Qinjun, XU Jingjing. Effects of 9-Cis-Retinoic Acid on the Proliferation and Apoptosis of Human Lung Cancer Cell Line A2[J]. Journal of Food Science and Biotechnology,2015,34(7):779-784.

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在线发布日期: 2015-11-28
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