聚乙烯醇降解菌的筛选与降解条件优化
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Screening of PVA-Degrading Strains and Optimization of Degradation Conditions
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    摘要:

    为了实现纺织工业废水中PVA1799的生物降解,从纺织印染厂区筛选到一个能快速降解培养基中PVA1799的混合菌系,命名为SH-TD。从中分离出3株纯培养菌株,分别命名鉴定为SH1 (Aeromonas sp.),TD5 (Pseudomonas sp.),TD33 (Acinetobacter sp.)。通过对含PVA1799出发培养基进行单因素及Placckett-Burman实验筛选出三个对PVA1799降解有显著影响的因素分别为Yeast-Extract、(NH4)2HPO4、CaCl2·2H2O。然后进行最陡爬坡实验逼近最佳响应面区域,最后通过Box-Behnken设计,利用Design-Expert软件进行回归分析,得到各因素的最佳质量浓度组成为(g/L):Yeast-Extract 0.04、(NH4)2HPO4 48.42、BaCl2 0.06。在最优条件下,24 h PVA降解率从原先的30%提高到80%。

    Abstract:

    In order to achieve the biodegradation of PVA1799 in the textile industrial waste water,an bacteria mixture with efficient degradation was screened and named as SH-TD. Three strains with PVA-degrading ability were isolated from the mixed culture SH-TD and nasmed as SH1(Aeromonas sp.),TD5(Pseudomonas sp.),and TD33(Acinetobacter sp.),respectively. The results from the single factor and Plackett-Burman experiments showed that Yeast-Extract,(NH4)2HPO4 and BaCl2 constituents have the significant influence on PVA degrading rate. Through ascent and Box-Behnken experiments,a response surface methodology(RSM) model was constructed. Data were analyzed by use of Design-Expert software. The optimal concentrations(g/L)of the variables were determined as (g/L):Yeast-Extract 0.04,(NH4)2HPO4 48.42,BaCl2 0.06. Under the optimal conditions,the PVA degradation rate after 24 h increased from 30% to 80% .

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李坤,李江华,刘龙,堵国成,陈坚.聚乙烯醇降解菌的筛选与降解条件优化[J].食品与生物技术学报,2015,34(8):835-841.

LI Kun, LI Jianghua, LIU Long, DU Guocheng, CHEN Jian. Screening of PVA-Degrading Strains and Optimization of Degradation Conditions[J]. Journal of Food Science and Biotechnology,2015,34(8):835-841.

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  • 在线发布日期: 2015-11-28
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