高粘附性戊糖片球菌的筛选、标记及其表面疏水与自凝聚性特征

doi:10.3969/j.issn.1673-1689.2015.09.005

首页 > 过刊浏览>2015年第34卷第9期 >926-934. DOI:10.3969/j.issn.1673-1689.2015.09.005

高粘附性戊糖片球菌的筛选、标记及其表面疏水与自凝聚性特征
DOI:
                        10.3969/j.issn.1673-1689.2015.09.005
                    
作者:
                        
                        
                    
作者单位:
作者简介:
通讯作者:
中图分类号:
基金项目:

Screening and Molecular Marking of Highly Adhesive Pediococcus pentosaceus and the Characteristics of Their Surface Hydrophobicities and Autoaggregation Abilities

Author:

Affiliation:

Fund Project:

摘要

图/表

访问统计

参考文献

相似文献

引证文献

资源附件

文章评论

摘要:

通过常规乳酸菌分离技术结合形态观察、生理生化试验，以及16S rDNA同源性分析等方法，从臭豆腐发酵卤水中分离获得7株戊糖片球菌分离株，并通过随机扩增多态性DNA技术构建了不同菌株的特征指纹图谱；以Caco-2细胞和固定化肠黏液蛋白质作为体外模型，研究了菌株的粘附能力，并探讨了菌株粘附能力与基因型以及表面疏水性、自凝聚能力等表型特征的相关性。结果显示，发酵卤水中的戊糖片球菌存在高度分子多样性，7个分离株中存在6种不同的指纹图谱模式，其中A5型菌株(F28-8和Y27-4)对Caco-2细胞和肠黏液蛋白质的粘附性最强，并显示了高度的疏水性(﹥90%)和较强的自凝聚能力(﹥25%)。相关性分析表明，戊糖片球菌表面疏水率和自凝聚率与Caco-2细胞粘附率测定结果呈显著正相关(r=0.900和0.792，P﹤0.05)，但是与肠黏液蛋白质粘附率测定结果相关性不显著(r=0.426和0.700，P﹥0.05)。研究成果为建立高粘附性戊糖片球菌快速筛选方法及其体内定植和分布研究提供了依据。

Abstract:

Seven strains of Pediococcus pentosaceus were isolated and identified from the fermented stinky tofu brine using methods of conventional bacteria isolation combined with physiological and biochemical test and 16S rDNA sequencing identification. The specific fingerprint for each strain was developed by the random amplified polymorphic DNA(RAPD) technique. The adhesive ability of the strain and the correlation of phenotypic characteristics of strain adhesion and genotype，surface hydrophobicity，and aggregation ability were studied using Caco-2 cell and intestinal mucus protein as in vitro model. Results showed that six different fingerprint patterns were found in the seven Pediococcus pentosaceus strains，among them，the A5 subtype of strains(F28-8 and Y27-4) showed the strongest adhesion to the Caco-2 cells and intestinal mucus. Furthermore，these two strains also exhibited highly hydrophobic(the hydrophobicity rate > 90%) and higher aggregation ability(the aggregation rate at 2 h > 5%). Correlation analysis results showed that the surface hydrophobicity and aggregation ability of Pediococcus pentosaceus were positively related to the adhesive ability to the Caco-2 cell significantly(r=0.900 and 0.792，P<0.05)，but they were not significantly correlated to the adhesive ability to the intestinal mucus(r = 0.426 and 0.700，P>0.05). The results of this study provide the basis for establishing techniques of rapid screening highly adhesive Pediococcus pentosaceus strains and their colonization and distribution in vivo.

参考文献

相似文献

引证文献

引用本文

杨振泉,靳彩娟,张咪,王晓霖,高璐,顾瑞霞.高粘附性戊糖片球菌的筛选、标记及其表面疏水与自凝聚性特征[J].食品与生物技术学报,2015,34(9):926-934.

YANG Zhenquan, JIN Caijuan, ZHANG Mi, WANG Xiaolin, GAO Lu, GU Ruixia. Screening and Molecular Marking of Highly Adhesive Pediococcus pentosaceus and the Characteristics of Their Surface Hydrophobicities and Autoaggregation Abilities[J]. Journal of Food Science and Biotechnology,2015,34(9):926-934.

复制

文章指标

点击次数:
下载次数:
HTML阅读次数:
引用次数:

历史

收稿日期:
最后修改日期:
录用日期:
在线发布日期: 2015-11-28
出版日期:

地址：江苏省无锡市蠡湖大道1800号邮政编码：214122

电话：0510-85913526 电子邮件：xbbjb@jiangnan.edu.cn

技术支持：北京勤云科技发展有限公司

引用本文

分享

文章指标

历史

微信公众号二维码

手机版网站二维码