“沪农灵芝1号”品种子实体多糖分析
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Analysis of Large Molecular Weight Polysaccharide in the Fruiting Bodies of Ganoderma lucidum Cultivar ‘Hunonglingzhi 1’
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    摘要:

    以水提醇沉法获得“沪农灵芝1号”子实体中的灵芝多糖,并对其结构特征和含量进行分析。高效凝胶尺寸排阻色谱-多角度激光光散射仪-示差折光检测仪联用分析结果表明,该多糖重均相对分子质量为2.28×106,均方根半径为150.1 nm,多分散系数(Mw/Mn)为1.14,在水溶液中呈棒状结构。单糖组成及甲基化分析结合酶解试验结果表明,该多糖为β-1,3/1,6-葡聚糖。经液相色谱法定量检测,段木栽培所得灵芝子实体中,该多糖质量分数为0.52 mg/g,显著高于袋料栽培所得子实体中相应组分的含量。

    Abstract:

    The high molecular polysaccharide fraction was purified by precipitating a hot-water extract with 20%(V/V) ethanol from fruiting bodies of Ganoderma lucidum cultivar 'Hunonglingzhi 1'. The high performance size exclusion chromatography - multiple angle laser light scattering - refractive index detector(HPSEC-MALLS-RI) was adopted to determine the molecular characteristics of this fraction. Results indicated that the polysaccharide possessed high weight average molecular weight(Mw) of 2.28×106,small polydispersity of 1.14 and large gyration radius(Rg) of 150.1 nm,and existed with a rigid chain conformation in the liquid solution. Structural analysis showed that this polysaccharide was a β-(1,3)/(1,6)-linked D-glucan. Its content determined by HPLC in wood cultivated fruiting bodies was about 0.52 mg/g,which was significantly higher than that in bag cultivated pattern.

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刘艳芳,唐庆九,张劲松,Steve W. Cui,杨焱,吴迪,周帅,唐传红,张忠.“沪农灵芝1号”品种子实体多糖分析[J].食品与生物技术学报,2015,34(10):1078-1082.

LIU Yanfang, TANG Qingjiu, ZHANG Jingsong, Steve W. Cui, YANG Yan, WU Di, ZHOU Shuai, TANG Chuanhong, ZHANG Zhong. Analysis of Large Molecular Weight Polysaccharide in the Fruiting Bodies of Ganoderma lucidum Cultivar ‘Hunonglingzhi 1’[J]. Journal of Food Science and Biotechnology,2015,34(10):1078-1082.

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  • 在线发布日期: 2016-01-30
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