微波辅助酶催化甜菊苷的转苷和水解
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Q684;O629.13;Q556.2

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Microwave-Assisted Enzymatic Transglycosylation and Hydrolysis of Stevioside
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    摘要:

    分别研究了微波辅助α-环糊精转移酶(α-CGTase)催化甜菊苷(St)转苷,和β-半乳糖苷酶催化St水解的反应。实验表明,微波能够加速来源于Paenibacillus macerans JFB05-01的α-CGTase催化St转苷反应,催化效率提高了21.7倍;但微波对β-半乳糖苷酶催化St的水解反应的影响较小。α-CGTase的加酶量为1 000 U/g时,反应1 min,St的转化率高达71.6%,St-Glc 1的产率为23.7%。

    Abstract:

    Microwave-assisted enzymatic transglycosylation and hydrolysis of stevioside (St) were investigated using α-cyclodextrin glucanotransferase (α-CGTase) and β-galactosidase,respectively. Microwave irradiation could accelerate the transglycosylation of St using α-CGTase extracted from Paenibacillus macerans JFB05-01,and the catalytic efficiency was increased by 21.7 folds. However,little effect was observed when microwave irradiation was applied to the enzymatic hydrolysis of St using β-galactosidase. With 1 000 U/g of α-CGTase was loaded,the conversion of St could reach as high as 71.6% after 1 min and the yield of St-Glc 1 was 23.7% correspondingly.

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万会达.微波辅助酶催化甜菊苷的转苷和水解[J].食品与生物技术学报,2015,34(12):1338-1343.

WAN Huida. Microwave-Assisted Enzymatic Transglycosylation and Hydrolysis of Stevioside[J]. Journal of Food Science and Biotechnology,2015,34(12):1338-1343.

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  • 在线发布日期: 2016-01-30
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