樟芝菌液态发酵合成安卓奎诺尔的条件优化
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Optimization of Submerged Fermentation for the Biosynthesis of Antroquinonol from Antrodia camphorata
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    摘要:

    安卓奎诺尔(Antroquinonol)是药食两用真菌樟芝子实体中的特征代谢产物之一,具有显著的生理活性。然而,在普通液态发酵条件下,樟芝菌丝体无法合成Antroquinonol,作者在添加前体物质和效应物促进樟芝液态发酵合成Antroquinonol的基础上,对其培养基以及发酵条件进行研究。结果表明,在复合添加大豆油和辅酶Q0的基础上,利用单因素实验分别对樟芝菌液态发酵培养基的碳氮源的种类和发酵条件进行了优化,最终确定发酵培养基的最佳碳源为米粉50 g/L,氮源为大豆粉6 g/L和玉米浆粉8 g/L。最适的发酵条件为初始pH 6,接种量为体积分数10%,发酵温度25 ℃,摇床转速150 r/min,在此条件下Antroquinonol的产量可以达到170.34 mg/L。

    Abstract:

    Antroquinonol,one of the metabolites in the edible and medicinal mushroom Antrodia camphorate,is considered as an effective bioactive component. However,Antroquinonol could not be biosynthesized via conventional submerged fermentation of A. camphorata. In this study,the culture medium and submerged fermentation conditions for the biosynthesis of Antroquinonol from A. camphorata was optimized based on the addition of precursors and effectors. The carbon/nitrogen source and the culture conditions of A. camphorata in submerged fermentation were optimized by single factor experiment with soybean oil and coenzyme Q0 added. The optimal carbon source was rice flour of 50 g/L,while the nitrogen source was soybean flour of 6 g/L and corn steep powder of 8 g/L. The initial pH is pH 6 with the inoculums concentration of 10% (v/v),and the fermentation temperature is 25 ℃ at 150 rpm,under which the production of Antroquinonol can reach 170.34 mg/L.

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胡永丹,路瑞秋,张薄博,许赣荣.樟芝菌液态发酵合成安卓奎诺尔的条件优化[J].食品与生物技术学报,2016,35(1):28-34.

HU Yongdan, LU Ruiqiu, ZHANG Bobo, XU Ganrong. Optimization of Submerged Fermentation for the Biosynthesis of Antroquinonol from Antrodia camphorata[J]. Journal of Food Science and Biotechnology,2016,35(1):28-34.

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  • 在线发布日期: 2016-03-11
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