脱脂米糠碱溶醇溶物抗氧化活性研究
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Antioxidant Activity of the Alkali and Alcohol Soluble Extracts from the Defatted Rice Bran
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    摘要:

    脱脂米糠的碱溶醇溶物具有良好的体外抗氧化活性。从脱脂米糠中提取抗氧化物,采用羟基自由基体系、DPPH自由基体系、超氧阴离子自由基体系、亚油酸体系、纯猪油体系、ORAC体系、PC12细胞氧化损伤体系,研究脱脂米糠的碱溶醇溶物清除自由基和脂质过氧化物的效果。结果表明:当样品质量浓度为0.2 g/mL时,其对·OH的清除率超过50%;样品质量浓度为0.6 mg/mL时,酶解产物的DPPH清除率可达90%;样品质量浓度45.0 mg/mL时,O2-清除率约为50%;样品浓度高于60 μg/mL时,样品的ORAC值与异VC钠相当;在亚油酸体系中,500 μg/mL的样品有明显的抗氧化效果,500 μg/mL和1 mg/mL的样品显著好于相同浓度的异VC钠;在猪油抗氧化体系中,质量分数5%样品添加组的猪油达到相同的pov所需的天数延长;随着样品质量浓度的增加,PC12细胞存活率呈现依次升高的趋势,5 mg/mL的样品的PC12细胞存活率达到82.2%,抗氧化效果好于200 μmol/L的Trolox。

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    The alkali and alcohol soluble extracts from the defatted rice bran exhibit a good antioxidant activity in vitro. The antioxidant effects of the extracted antioxidants from the defatted rice bran on scavenging free radicals and lipid peroxides aganist oxidative damage system were studied by hydroxyl radical system,DPPH radical system,superoxide anion radical system,linoleic acid system,pure lard system,ORAC system,and PC12 cells,respectively. The scavenging rate of ·OH was over 50% for the extracts of 0.2 g/mL,while the clearance rate of DPPH was as high as 90% for 0.6 mg/mL extracts. The O2- clearance rate is about 50% for the extracts of 45.0 mg/mL and the ORAC value reached a comparable value with sodium D-isoascorbate when the extracts concentration was larger than 60 μg/mL,In linoleic acid system,a significant antioxidant activity was observed for the extracts of 500 μg/mL. At the meanwhile,the effect was better than sodium D-isoascorbate with same concentration for the extracts of 500 μg/mL and 1 mg/mL. In lard antioxidant studies,a longer duration was required for pov growing to the same level if the lard samples added with 5% extracts. The PC12 cell viability presented a successively increasing trend when the sample concentration increased,for example,the PC12 cell viability reached to 82.2% with 5 mg/mL sample added and the antioxidant effect was better than Trolox of 200 μmol/L.

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崔红军,徐宝才,王莉,韩衍青,罗小虎.脱脂米糠碱溶醇溶物抗氧化活性研究[J].食品与生物技术学报,2016,35(1):35-41.

CUI Hongjun, XU Baocai, WANG Li, HAN Yanqing, LUO Xiaohu. Antioxidant Activity of the Alkali and Alcohol Soluble Extracts from the Defatted Rice Bran[J]. Journal of Food Science and Biotechnology,2016,35(1):35-41.

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  • 在线发布日期: 2016-03-11
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