酶解条斑紫菜制备抗氧化肽及综合利用
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Study on the Preparation of Antioxidative Peptides from Enzymatic Hydrolysis of Laver and Their Comprehensive Utilization
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    摘要:

    将酶解反应与几种膜分离技术集成构建一种制备紫菜抗氧化肽,并同时分级分离其它活性物质以实现综合利用。先后采用截留相对分子质量10 000的超滤膜和500的纳滤膜对紫菜酶解液进行分离纯化,优化操作参数,抗氧化肽回收率达73.1%,多糖回收率达55%,膳食纤维回收率达85%,多肽得率达18%;考察该工艺所得紫菜抗氧化多肽的应用特性,结果表明:纳滤浓缩后多肽液0.5 mg/mL对DPPH自由基清除率可达68.5%,该多肽最适作用pH为8.5,经80 ℃处理6 h后的紫菜抗氧化肽仍保留83%左右的相对DPPH自由基清除率。

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    This study integrate enzymatic reaction with several membrane technologies to construct a method for comprehensive utilization of antioxidant peptide and other active substances from laver. The hydrolysate of laver was separated and purified through ultrafiltration(10 kDa cut-off molecular weight) and nanofiltration(500 Da cut-off molecular weight). After optimizing the operation parameters of membrane separation,recovery rates of antioxidant peptide,polysaccharide,dietary fiber,and peptide reached 73.1%,55%,85%,and 18%,respectively. The peptides from laver showed the DPPH scavenging activity. The DPPH scavenging activity of 0.5 mg/mL nanofiltration peptide solution was up to 68.5%. Furthermore,the optimum pH value of laver antioxidant peptide was 8.5. Meanwhile,these peptides showed a good stability,it retained about 83% relative scavenging activity of DPPH radical after heated at 80 ℃ for 6 h.

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刘冬冬.酶解条斑紫菜制备抗氧化肽及综合利用[J].食品与生物技术学报,2016,35(2):166-172.

LIU Dondong. Study on the Preparation of Antioxidative Peptides from Enzymatic Hydrolysis of Laver and Their Comprehensive Utilization[J]. Journal of Food Science and Biotechnology,2016,35(2):166-172.

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  • 在线发布日期: 2016-10-25
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