应用嗜热四膜虫评估苯甲酸钠的生物毒性

doi:10.3969/j.issn.1673-1689.2016.05.011

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应用嗜热四膜虫评估苯甲酸钠的生物毒性
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                        10.3969/j.issn.1673-1689.2016.05.011
                    
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Application Tetrahymena thermophila for Assessment Biotoxicity of Sodium Benzoate

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应用2株嗜热四膜虫（Tetrahymena thermophila）Cu428.2株和B2086.2株评估了食品防腐剂苯甲酸钠（sodium benzoate，SB）的生物毒性，探讨嗜热四膜虫在食品安全监测中的应用。在SB质量分数为0%，0.01%，0.05%，0.1%，0.25%，0.5%，作用6 h，观察嗜热四膜虫的细胞形态和运动情况。结果表明：SB质量分数为0.01% 和0.05%时，两株嗜热四膜虫在Neff培养基中的活动均明显降低，细胞形态发生皱缩，细胞变小；SB质量分数为0.1%～0.5%时，两株嗜热四膜虫细胞形态明显由椭圆形变成圆形，细胞膜出现孔洞，甚至出现破裂死亡细胞，质量分数越高，细胞膜损伤越大，凋亡细胞数量越多。嗜热四膜虫培养于Neff培养基中，分别添加终质量分数为0%，0.000 1%， 0.001%，0.005%，0.01%，0.05%的 SB，测定添加SB后嗜热四膜虫的生长曲线及SB作用24 h的SOD活力，评估SB对嗜热四膜虫的种群数量和SOD活力的影响。结果表明：随着SB质量分数的增加、嗜热四膜虫的种群增长速度降低，而0.05%SB对嗜热四膜虫具有杀灭作用，但0.000 1%SB对嗜热四膜虫种群的增长有一定的促进作用。嗜热四膜虫的SOD活力基本上随着SB质量分数的增加而降低，然而低质量分数的SB可使SOD活力在一定时间内增强。

Abstract:

For investigation the application of protozoan Tetrahymena thermophila on food safety detection，two strains of protozoan T. thermophila Cu428.2 and B2086.2 were used as substrate to study the biotoxicity of food preservative sodium benzoate（SB）. Effect of SB on T. thermophila was assessed from changes in cell shape，movement style，population growth and superoxide dismutase（SOD） activity after adding SB to the culture of T. thermophila in Neff medium. Cell morphology and movement of T. thermophila were observed under inverted microscope at 6 h after adding 0%，0.01%，0.05%，0.1%，0.25%，0.5%SB to the culture. The results showed that in 0.01% and 0.05% SB，both strains of T. thermophila cells became shrunken and smaller，the speed of cell movement in the media was significantly slower than the control group. While in 0.1%~0.5% SB，cells morphology turned round from oval，holes appeared in the cell membrane，rupture and death cells were found. The higher the concentration of SB was，the greater damage of the cell membrane and the more the apoptotic cells appeared. Population growth curve and 24 h SOD activity of the two strains of T. thermophila cultured in Neff medium were determined in 0.000 1%，0.001%，0.005%，0.01% ，0.05% SB compared to the control group. The results suggested with the increase of SB concentration，population growth rate of T.thermophila decreased and 0.05% SB even killed the original seeded cells，however 0.000 1% SB promoted the population growth. While as the SB concentration increased ，the activity of T. thermophila SOD basically decreased，however，lower concentration of SB could enhanced the activity of SOD in a certain period of time.

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潘厚军,张銮妫,梁慧丽,李莹莹,王英英,林强,石存斌,吴淑勤.应用嗜热四膜虫评估苯甲酸钠的生物毒性[J].食品与生物技术学报,2016,35(5):517-524.

PAN Houjun, ZHANG Luangui, LIANG Huili, LI Yingying, WANG Yingying, LIN Qiang, SHI Cunbin, WU Shuqin. Application Tetrahymena thermophila for Assessment Biotoxicity of Sodium Benzoate[J]. Journal of Food Science and Biotechnology,2016,35(5):517-524.

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