屎肠球菌和枯草芽孢杆菌混合培养条件的优化
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Study on Mixed Culture of Enterococcus faecium and Bacillus subtilis
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    摘要:

    以屎肠球菌和枯草芽孢杆菌为研究对象,以MRS培养基为基础培养基,采用单因素试验、Box-Behnken 实验设计对屎肠球菌和枯草芽孢杆菌的混合培养进行优化。结果表明:初始pH、装液量和氮源对活菌数影响显著。最优条件为:初始pH 6.5、装液量50/500 mL、氮源用量35 g/L、接种比1∶12、转速180 r/min,在此条件下活菌数达到6.99×1010 CFU/mL,比优化前提高了5倍,屎肠球菌菌株和枯草芽孢杆菌菌株的活菌数分别达到4.58×1010 CFU/mL和2.41×1010 CFU/mL。

    Abstract:

    In order to investigate the effect of Enterococcus faecium and Bacillus subtilis mixed culture on their biomass,MRS medium,single factor experiment,Box-Behnken Design were adopted to optimize the mixed culture. Results showed that medium initial pH,liquid volume and nitrogen source had significant effects on the viable counts of the strains. The optimal culture conditions were as follows:initial medium pH 6.5,50 mL liquid medium in 500 mL flask with 180 rpm in shake,35 g/L of nitrogen source,the inoculation of Enterococcus faecium and Bacillus subtilis at a ratio of 1∶12. Under this conditions,the total viable counts reached to 6.99×1010 CFU/mL,with 4.58×1010 CFU/mL of Enterococcus faecium and 2.41×1010 CFU/mL of Bacillus subtilis,which was five times higher than that of before optimization.

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胡治铭,梁丛丛,国洪旭,邓先余.屎肠球菌和枯草芽孢杆菌混合培养条件的优化[J].食品与生物技术学报,2016,35(5):537-542.

HU Zhiming, LIANG Congcong, GUO Hongxu, DENG Xianyu. Study on Mixed Culture of Enterococcus faecium and Bacillus subtilis[J]. Journal of Food Science and Biotechnology,2016,35(5):537-542.

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  • 在线发布日期: 2016-11-01
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