实时荧光PCR法检测食品中梅花鹿成分

doi:10.3969/j.issn.1673-1689.2016.10.012

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实时荧光PCR法检测食品中梅花鹿成分
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                        10.3969/j.issn.1673-1689.2016.10.012
                    
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中图分类号:R817.93；TS207
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Detection for Sika Deer-Origin Ingredients in Foods by Fluorescent Real-Time PCR Method

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摘要:

采用实时荧光聚合酶链式反应技术检测食品中梅花鹿成分。通过对梅花鹿基因序列对比分析，选取种间差异大、种内差异小的线粒体细胞色素C氧化酶亚基Ⅰ基因序列作为靶序列，利用Primer Express、Oligo和DNAMAN等软件设计了梅花鹿特异性的引物和TaqMan探针，并用已知样本对引物和TaqMan探针的物种特异性、检测灵敏度进行验证和检测。实验结果表明，引物和探针对梅花鹿成分检测的特异性良好，与其他物种DNA无非目标扩增，对梅花鹿DNA的扩增效率为91.68%，该方法的检测灵敏度达0.42 pg/反应。

Abstract:

A method based on fluorescent real-time polymerase chain reaction was developed to detect sika deer-ingredient in food products. By comparative analysis of sika deer gene sequences，sensitive primers and TaqMan probe were designed on a conserved region of mitochondrial cytochrome c oxidase subunit I sequence，using the Primer Express，Oligo and DNAMAN software. Furthermore，the species specificity and detection sensitivity were tested and validated by using standard samples. Results indicated that primers and TaqMan probe have a high sensitivity for sika deer without non-targeted amplification for other species DNA. Sensitivity of detection was confirmed to be 0.42 pg per reaction with a 91.68% amplification efficiency. Real-time PCR method was applied to detect sika deer meat in the market. Compared with the standard method，this method was more rapid，accurate and convenient，which was suitable for fast authentication of sika deer-origin ingredients.

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齐春萌,杨昕霆,薛晨玉,赵琳娜,侯彩云.实时荧光PCR法检测食品中梅花鹿成分[J].食品与生物技术学报,2016,35(10):1088-1092.

QI Chunmeng, YANG Xinting, XUE Chenyu, ZHAO Linna, HOU Caiyun. Detection for Sika Deer-Origin Ingredients in Foods by Fluorescent Real-Time PCR Method[J]. Journal of Food Science and Biotechnology,2016,35(10):1088-1092.

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在线发布日期: 2016-10-31
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