谷氨酰胺转胺酶诱导交联发酵乳蛋白的体内抗氧化功效

doi:10.3969/j.issn.1673-1689.2016.12.015

首页 > 过刊浏览>2016年第35卷第12期 >1330-1335. DOI:10.3969/j.issn.1673-1689.2016.12.015

谷氨酰胺转胺酶诱导交联发酵乳蛋白的体内抗氧化功效
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                        10.3969/j.issn.1673-1689.2016.12.015
                    
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Anti-Oxidative Activity of Microbial Transglutaminase Cross-Linked Fermentation Milk Protein in vivo

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为了研究微生物谷氨酰胺转胺酶诱导交联发酵乳蛋白（Microbial Transglutaminase Cross-Linked Fermentation Milk Protein，mTG-FPM）对D - 半乳糖（D-gal）衰老模型小鼠的抗氧化活性的影响，试验设正常组、D-gal模型组、D-gal＋0UmTG-FPM组、D-gal＋1UmTG-FPM组、D-gal＋3UmTG-FPM组和D-gal＋VE阳性对照组共6组，每组10只C57BL/J小鼠。采用皮下连续注射D-Gal方式建立衰老小鼠模型。造模同时，FPM各组每天灌服1.5 g/kg mTG-FPM/FPM，D-gal +VE阳性对照组每天灌服100 mg/kg 的VE，模型对照和正常组每天灌服蒸馏水0.2 mL/10 g。连续灌胃8 w后，测定各组小鼠肝肾组织和血清中的相关抗氧化指标（CAT、SOD、GSH-Px MDA）。结果表明：D-Gal可显著降低衰老模型小鼠肝脏和血清中CAT及GSH-Px活性（P ＜ 0.01，P ＜ 0.05）；显著降低肾脏和血清中的SOD活力（P ＜ 0.01，P ＜ 0.05），显著升高MDA含量（P ＜ 0.01）。与模型组和0UmTG组相比，3UmTG组的肝脏CAT，1UmTG组血清CAT活性和3U mTG组肝脏GSH-Px活性显著升高（比0UmTG组分别升高11.14%、35.57%和22.36%，P ＜ 0.01，P ＜ 0.05）；而3UmTG组肾脏中的MDA含量显著降低（比0UmTG组降低26.41%，P ＜ 0.05）。因此，mTG诱导交联处理可在一定程度上改善FPM的体内抗氧化活性。

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The study was carried out to investigate the anti-oxidative effect of Microbial Transglutaminase Cross-Linked Fermentation Milk Protein（mTG-FPM） in aging mice induced by D-galactose（D-gal）. Sixty C57BL/6J female mice（12 w old）were divided into 6 groups including the control group，D-gal model group，D-gal-induced mice treated with FPM cross-linked mTG by 0 U，1 U and 3 U/g protein（as D-gal+0 UmTG-FMP，D-gal+1UmTG-FMP and D-gal+3 UmTG-FMP groups），at 1.5 g/kg bw via ig，and D-gal-induced mouse treated with vitamin E as the positive group at 100 mg/kg via ig，respectively. After continuous administration for 8 weeks，the anti-oxidative parameters（CAT，SOD，MDA GSH-Px） in liver，kidney tissues，and serum of mice in each group were determined. The results showed that mice induced by D-gal had lower CAT and GSH-Px activity in liver and serum，lower SOD activity of kidney and serum，and higher MDA content of kidney and serum，when compared with the normal mice（P < 0.01，P < 0.05）. Compared to the model and untreated FPM groups，FPM treated with mTG had higher CAT activities in liver tissue（3 U mTG treatment，P < 0.01） and in serum（1 U mTG treatment，P < 0.05） up to 11.14% and 35.57% respectively，whereas the content of MDA in kidney tissue（P < 0.01） in FPM treated with mTG was reduced 26.41%. The activity of GSH-Px in liver tissue was also sharply higher（22.36% increase） in D-gal+3 U mTG-FPM group than that of in FPM group untreated with mTG（P < 0.01）. It was concluded that the mTG Cross-linked effect in FPM can improve the anti-oxidative ability of aging mice induced by D-gal.

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吕建敏,袁海娜.谷氨酰胺转胺酶诱导交联发酵乳蛋白的体内抗氧化功效[J].食品与生物技术学报,2016,35(12):1330-1335.

LU Jianmin, YUAN Haina. Anti-Oxidative Activity of Microbial Transglutaminase Cross-Linked Fermentation Milk Protein in vivo[J]. Journal of Food Science and Biotechnology,2016,35(12):1330-1335.

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