海洋细菌Planococcus rifietoensis发酵产色素培养基的优化
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

Q815

基金项目:


Optimization of Fermentation Medium for the Production of Pigments by Marine Bacteria Planococcus rifietoensis
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    在单因素的基础上,以Plackett-Burman(PB)设计结合响应面(RSM)分析法,对产色素海洋细菌Planococcus rifietoensis发酵培养基的8个营养成分配比进行优化。结果表明:培养基最佳碳氮源分别是葡萄糖和蛋白胨,葡萄糖、MgSO4·7H2O和酵母粉添加量显著影响色素的产量。Box-Benhnken设计分析确定最优培养基配方为:葡萄糖9.78 g/L,蛋白胨 8.00 g/L,酵母粉2.05 g/L,MgSO4·7H2O 8.17 g/L,Na2HPO4 0.1 g/L,FeSO4·7H2O 0.005 g/L,NaCl 10 g/L,CaCl2 0.1 g/L,培养54 h,在此培养条件下,色素的OD值可达0.984,比优化前提高了245%。

    Abstract:

    Based on monofactorial experiments,Plackett-Burman(PB) design and response surface methodology(RSM) were applied to optimize 8 medium components for the production of pigments by marine bacteria strain Planococcus rifietoensis. Results showed that optimal carbon and nitrogen sources of the medium were glucose and peptone,respectively,and glucose,yeast extract and MgSO4·7H2O had major effects on the production of pigments. The optimal culture medium was comprised of glucose 9.78 g/L,peptone 8.00 g/L,yeast extract 2.05 g/L,MgSO4·7H2O 8.17 g/L,Na2HPO4 0.1 g/L,FeSO4·7H2O 0.005 g/L,NaCl 10 g/L and CaCl2 0.1 g/L. The incubation time was 54 hours. Under these conditions,the optical density(OD) of pigments reached to 0.984,which was 245% higher than that before optimization.

    参考文献
    相似文献
    引证文献
引用本文

孔菲,蔡宇杰,许晨,许建中,夏金梅.海洋细菌Planococcus rifietoensis发酵产色素培养基的优化[J].食品与生物技术学报,2017,36(2):179-186.

KONG Fei, CAI Yujie, XU Chen, XU Jianzhong, XIA Jinmei. Optimization of Fermentation Medium for the Production of Pigments by Marine Bacteria Planococcus rifietoensis[J]. Journal of Food Science and Biotechnology,2017,36(2):179-186.

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2017-04-28
  • 出版日期:

版权所有:《食品与生物技术学报》编辑部

地址:江苏省无锡市蠡湖大道1800号  邮政编码:214122

电话:0510-85913526  电子邮件:xbbjb@jiangnan.edu.cn

技术支持:北京勤云科技发展有限公司

微信公众号二维码

手机版网站二维码