载纳米银丝蛋白/海藻酸钠海绵的制备及表征
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R318.08

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Preparation and Characterization of Nano-Silver Loaded Fibroin/Alginate Sponge
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    摘要:

    以丝蛋白(SF)和海藻酸钠(SA)为基质构建新型负载纳米银生物敷料。以SF溶液为稳定剂,采用紫外光照射法合成了纳米银,加入SA后,经氯化钙/乙醇/水(摩尔比,1∶71∶77)处理3 h,将上述材料润洗冻干后得到负载纳米银的SF/SA复合海绵(AgNPs-SF/SA)。透射电子显微镜(TEM)观察显示,合成的纳米银平均粒径为(5.03±1.42) nm,在丝蛋白溶液中分布均匀。扫描电子显微镜(SEM)图像显示,与纯丝蛋白海绵相比,AgNPs-SF/SA显示连通的多孔结构。SA的加入增强了海绵的吸水性和保水性;在SF和SA的协同作用下,AgNPs-SF/SA的水蒸气透过率有所提高。以K-B扩散法和菌液培养法测定了AgNPs-SF/SA的抗菌性,结果显示,AgNPs-SF/SA对金黄色葡萄球菌和铜绿色假单胞菌的抗菌性优于阳性对照AQUACEL?誖Ag;24 h和48 h的菌液培养显示,AgNPs-SF/SA具有更持久的抗菌活性。综上所述,AgNPs-SF/SA的物理性能和抗菌性能优良,有望作为一种新型的抗菌生物敷料用于预防和治疗创面感染。

    Abstract:

    This study aimed to fabricate a novel Ag nano-particles(AgNPs) loaded biologic dressing based on silk fibroin(SF) and sodium alginate(SA). AgNPs were first synthesized in the stabilizer,SF solution,under UV irradiation,and after the addition of SA,were then treated by CaCl2/ethanol /water(molar ratio,1∶71∶77) for 3 h. After rinsing and freeze-drying,AgNPs loaded SF/SA composite sponge(AgNPs-SF/SA) was obtained. Transmission electron microscopy observation(TEM) showed that AgNPs were evenly distributed in the SF solution with a mean diameter of 5.03 nm. Scanning electron microscopy(SEM) images showed that compared with pure SF sponge,AgNPs-SF/SA had a linked porous structure. Addition of SA improved the water absorption and retention ability of the sponge and the synergistic effect of SF and SA increased the water vapor transmission rate. K-B disk diffusion and bacterial solution culture methods indicated that AgNPs-SF/SA possessed a higher and more continuous anti-bacterial potential than the positive control,AQUACEL?誖 Ag,against P. aeruginosa and S. aureus. In conclusion,the excellent physical and anti-bacterial properties demonstrated that AgNPs-SF/SA could be a novel anti-bacterial biologic dressing for prevention and treatment of wound infection.

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陈雍,赵朋,邓超,陈敬华.载纳米银丝蛋白/海藻酸钠海绵的制备及表征[J].食品与生物技术学报,2017,36(2):200-206.

CHEN Yong, ZHAO Peng, DENG Chao, CHEN Jinghua. Preparation and Characterization of Nano-Silver Loaded Fibroin/Alginate Sponge[J]. Journal of Food Science and Biotechnology,2017,36(2):200-206.

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  • 在线发布日期: 2017-04-28
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