芽胞漆酶的固定化方法对比研究与载体选择
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

Q814.2

基金项目:


Immobilization of Spore Laccase: Comparative Studies and Carriers Selection
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    为了找到适合芽胞漆酶固定化的载体和方法,对12种载体共19种芽胞漆酶固定化方案进行了对比研究,并通过参考固定化芽胞漆酶的酶活力回收率、重复利用率、成本等因素,选定以DEAE-纤维素为载体通过离子结合法固定为最佳方案。结果表明,包埋法固定化芽胞漆酶存在严重的扩散限制作用,部分包埋法制备的微球还会伴有溶解、膨胀的现象。而多孔材料常因孔径过小难以固定芽胞。芽胞以碳二亚胺为交联剂与载体进行共价结合,具有良好的固定化性能,可作为备选方案。

    Abstract:

    The comparative studies for immobilization of spore laccase were carried out using 19 different methods and 12 carriers. The optimal carrier and method were determined by the activity yield,reusability,and cost of the immobilized spore laccase. The ionic bonding method using DEAE-cellulose as the carrier was selected as the best method. The strong diffusion limitations were observed when the spore laccase was immobilized by embedding method. The microspheres might dissolve and swell if semi-embedding method was used. The small pore size made it difficult for porous materials to immobilized spore laccase. The excellent immobilization properties were achieved when the spores were covalently bound to the carriers using carbodiimide as the crosslinker. It was thus recommended as an alternative method.

    参考文献
    相似文献
    引证文献
引用本文

周稳,管政兵,蔡宇杰,赵宏,廖祥儒.芽胞漆酶的固定化方法对比研究与载体选择[J].食品与生物技术学报,2017,36(4):363-369.

ZHOU Wen, GUAN Zhengbing, CAI Yujie, ZHAO Hong, LIAO Xiangru. Immobilization of Spore Laccase: Comparative Studies and Carriers Selection[J]. Journal of Food Science and Biotechnology,2017,36(4):363-369.

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2017-05-05
  • 出版日期:

版权所有:《食品与生物技术学报》编辑部

地址:江苏省无锡市蠡湖大道1800号  邮政编码:214122

电话:0510-85913526  电子邮件:xbbjb@jiangnan.edu.cn

技术支持:北京勤云科技发展有限公司

微信公众号二维码

手机版网站二维码