艾蒿多糖抑菌活性及稳定性
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Study on Antimicrobial Activities of Polysaccharides from Artemisia argyi and Its Stability
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    摘要:

    采用水提法提取艾蒿多糖,用滤纸片法测定多糖的抑菌活性及最低抑菌浓度(MIC),同时研究艾蒿多糖抑菌活性的酸碱稳定性、热稳定性、紫外稳定性。结果表明:艾蒿多糖对3种常见细菌均有一定的抑制效果,对革兰氏阳性细菌如金黄色葡萄球菌和枯草芽孢杆菌抑制作用较强,对革兰氏阴性菌大肠杆菌的抑制作用较弱;艾蒿多糖对枯草芽孢杆菌、金黄色葡萄球菌、大肠杆菌的最小抑制质量浓度(MIC) 分别为0.625、0.625、1.25 mg/mL;多糖的抑菌活性对温度和紫外照射具有较好稳定性,在不同的pH 值条件下抑菌活性相对不稳定,酸性处理条件下抑菌活性较强,碱性处理条件下抑菌活性较弱。艾蒿多糖具有良好的抑菌活性,可用于天然食品防腐剂的开发利用。

    Abstract:

    Artemisia argyi polysaccharide extracted by hot water and measurement the antibacterial activity and minimum inhibitory concentration(MIC) of polysaccharides by using the filter paper method,also studied the pH stability,thermal stability,UV stabilizers stability of Artemisia polysaccharide antibacterial activity. The results showed that:Artemisia argyi polysaccharides have a certain inhibitory effect on three common bacteria,and have strong effect on gram-positive bacteria Staphylococcus aureus and Bacillus subtilis,but the effect on gram-negative bacteria E. coli is weak.The MICs of the polysaccharide agaginst Bacillus subtilis,Staphylococcus aureus and Escherichia coli were 0.625、0.625、1.25 mg/mL respectively. The antimicrobial activities of the polysaccharide were stable under the conditions of heat and ultraviolet ray,but were unstable under different pH values,strong in acid and weak in base. Artemisia argyi polysaccharide has good antibacterial activity,it can be used for the development and utilization of natural food preservatives.

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孙义玄,包怡红.艾蒿多糖抑菌活性及稳定性[J].食品与生物技术学报,2017,36(9):990-995.

SUN Yixuan, BAO Yihong. Study on Antimicrobial Activities of Polysaccharides from Artemisia argyi and Its Stability[J]. Journal of Food Science and Biotechnology,2017,36(9):990-995.

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  • 在线发布日期: 2017-10-30
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