牛奶中残留麻保沙星的微生物学检测方法
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S859.84

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Determination of Marbofloxacin Residues in Milk by Microbiological Method
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    摘要:

    建立牛奶中残留麻保沙星的微生物学检测方法。试验以金黄色葡萄球菌为敏感菌,采用滤纸片法进行测定,以牛奶为样设计了添加回收试验以证实该方法的有效性。磷酸盐缓冲溶液和牛奶基质麻保沙星标准曲线的工作范围是0.1~1.6 μg/mL,最低检测限为 0.1 μg/mL。对纯牛奶样的3种添加水平,平均回收率范围在77.3%~93.3%之间,变异系数小于7.26%,该方法可以用于牛奶中麻保沙星残留的检测。

    Abstract:

    To establish a microbiological method for determination of marbofloxacin residues in milk. Marbofloxacin residues in milk was tested using filter paper method based on Staphylococcus selected as a sensitive bacteria,and then the recoveries were performed on milk samples to verify the feasibility of this method. Results:the data showed that the linear range for mornitoring marbofloxacin was from 0.1 μg/mL to 1.6 μg/mL based on phosphate buffer solution and milk matrix. The minimum limit detections of marbofloxacin in phosphate buffer solution and milk matrix were 0.1 μg/mL. The average recovery rates were in the range of 77.3%~93.3% and variation coefficient were less than 7.26% for three spiked levels of Erie milk samples. The obtained method can be feasible to mornitoring marbofloxacin residues in milk.

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陈秀金,任国艳,许凯旋,崔璨,张永丽,侯玉泽,胥传来,李根.牛奶中残留麻保沙星的微生物学检测方法[J].食品与生物技术学报,2018,37(1):107-111.

CHEN Xiujin, REN Guoyan, XU Kaixuan, CUI Can, ZHANG Yongli, HOU Yuze, XU Chuanlai, LI Gen. Determination of Marbofloxacin Residues in Milk by Microbiological Method[J]. Journal of Food Science and Biotechnology,2018,37(1):107-111.

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  • 在线发布日期: 2018-03-08
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