响应面法优化弹簧糊精的酶解制备工艺
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Application of Response Surface Methodology to Optimize Spring Dextrin Preparation Parameters by Enzyme Hydrolysis
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    摘要:

    以蜡质玉米淀粉为原料,通过单因素试验和响应面设计法,优化了普鲁兰酶酶解制备弹簧糊精的工艺参数,并建立数学模型,得到的最佳工艺参数为:pH 4.96、酶解温度53.32 ℃、酶用量420 U/g、酶解时间6 h,此工艺条件下制备的弹簧糊精纯度为99.2%。测定了制备的弹簧糊精的链长分布,实验结果表明聚合度(以DP表示)小于60的弹簧糊精占总弹簧糊精质量分数的95.5%,其中聚合度为DP 6~12、13~24、25~36、37~60的弹簧糊精质量分数分别为24.40%、45.08%、19.60%及10.92%。

    Abstract:

    The spring dextrin were prepared by enzymatic hydrolysis of waxy maize starch with pullulanase alone,and the process parameters were optimized by one-factor-at-a-time method and response surface methodology. The optimal results were observed at conditions of enzyme dosage 420 U/g,hydrolysis for 6 h at 53.32 ℃,pH 4.96,predicted using mathematical predictive model. Under these conditions,the spring dextrin purity was 99.2%. The chain-length distribution of the prepared spring dextrin were also evaluated. Results showed that the proportion of spring dextrin with DP<60 were 95.5%,and the relative contents of spring dextrin with DP6~12,DP13~24,DP25~36 and DP37~60 were 24.40%,45.08%,19.60% and 10.92%,respectively.

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闵丹丹,徐学明,焦爱权,潘小卫,金征宇.响应面法优化弹簧糊精的酶解制备工艺[J].食品与生物技术学报,2018,37(7):701-706.

MIN Dandan, XU Xueming, JIAO Aiquan, PAN Xiaowei, JIN Zhengyu. Application of Response Surface Methodology to Optimize Spring Dextrin Preparation Parameters by Enzyme Hydrolysis[J]. Journal of Food Science and Biotechnology,2018,37(7):701-706.

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  • 在线发布日期: 2018-09-28
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