重组Bacillus stearothermophilus β-CGTase在β-环糊精制备中的应用条件优化

doi:10.3969/j.issn.1673-1689.2018.09.001

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重组Bacillus stearothermophilus β-CGTase在β-环糊精制备中的应用条件优化
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                        10.3969/j.issn.1673-1689.2018.09.001
                    
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Studies on Preparation of β-Cyclodextrin Production Using Bacillus stearothermophilus β-Cyclodextrin Glycosyltransferase and Reaction Conditions Optimization

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以作者所在实验室前期构建的产嗜热脂肪芽孢杆菌β-环糊精葡萄糖基转移酶（β-CGTase）重组短小芽孢杆菌作为菌种，经过摇瓶发酵，得到酶活为49 U/mL的β-CGTase粗酶液。以马铃薯淀粉为底物用β-CGTase进行单酶法转化制备β-环糊精，并对转化条件进行优化。结果表明，最优反应条件为：反应时间18 h，初始pH 6.0，反应温度50 ℃，底物质量浓度15 g/dL，加酶量13 U/g底物。在最优条件下，总转化率最高为73.9%，其中β-环糊精比例为98.7%。在此基础上，建立了采用普鲁兰酶与β-CGTase复配同步转化淀粉制备β-环糊精的新工艺，并且优化了普鲁兰酶的加酶量。结果表明，当普鲁兰酶加量为50 U/g淀粉底物，反应时间18 h时，总转化率达到最高81.4%，其中β-环糊精占比97.7%。

Abstract:

Previously，a recombinant Brevibacillus sp. producing the Bacillus stearothermophilus cyclodextrin glycosyltransferase was constructed. In this study，the strain was cultured in shake flask for 48 h，it showed that the highest extracellular enzyme activity was 49 U/mL. The supernatant of the medium was used as crude enzyme for the production of β-cyclodextrin. Then the reaction conditions，such as initial pH，reaction temperature，starch concentration，CGTase dosage，pullulanase dosage and reaction time，on the yields of β-cyclodextrin was investigated. The optimized conditions for the conversion as the following：the initial pH was 6.0，the reaction temperature was 50 ℃，initial starch concentration was 150 g/L ，CGTase concentration was 13 U/mL，pullulanase concentration was 50 U/g starch. Under this conditions，the reaction was continued for 18 h，thecyclodextrin yield is 81.4% and the proportion of β-cyclodextrin is 97.7%.

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范博望,段绪果,吴敬.重组Bacillus stearothermophilus β-CGTase在β-环糊精制备中的应用条件优化[J].食品与生物技术学报,2018,37(9):897-902.

FAN Bowang, DUAN Xuguo, WU Jing. Studies on Preparation of β-Cyclodextrin Production Using Bacillus stearothermophilus β-Cyclodextrin Glycosyltransferase and Reaction Conditions Optimization[J]. Journal of Food Science and Biotechnology,2018,37(9):897-902.

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在线发布日期: 2018-10-30
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