杨树桑黄胞外多糖的分子结构及抗氧化活性

doi:10.3969/j.issn.1673-1689.2018.09.007

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杨树桑黄胞外多糖的分子结构及抗氧化活性
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                        10.3969/j.issn.1673-1689.2018.09.007
                    
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Molecular Structure and Antioxidant Activity of Polysaccharides from Phellinus vaninii Ljup

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对杨树桑黄胞外多糖（EPS）的相对分子质量、结构及抗氧化活性进行了研究。利用凝胶过滤法分离纯化EPS并测定多糖的相对分子质量，红外分析EPS结构的异头碳类型，GC/MS分析EPS的单糖组分，SEC/MALLS结合粘度法分析EPS的分子构象，利用·OH自由基和·DPPH自由基的清除率测定EPS的抗氧化活性。结果表明：杨树桑黄EPS分离纯化得到两个组分（Fr-I和Fr-II），其相对分子质量分别为627 500和55 000；红外分析可知，Fr-I为含有α-和β-构型共存的吡喃型甘露糖苷酸性杂多糖，Fr-II为含有α-吡喃型甘露糖苷酸性杂多糖；气质分析结果表明，Fr-I和Fr-II所含单糖组分为核糖、木糖、葡萄糖醛酸、半乳糖、葡萄糖、甘露糖和半乳糖醛酸；SEC/MALLS结果表明，EPS分散性很低，在水溶液中以球形构象存在，是一种高度紧密且具有分支结构的多糖聚集体。抗氧化测定表明，EPS质量浓度为10 mg/mL时，对·OH自由基的清除率为38.58%；质量浓度为5 mg/mL时，对·DPPH自由基的清除率高达59.17%，表现出良好的抗氧化活性。

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The molecular weight，structure and antioxidant activity of extracellular polysaccharide（EPS） from Phellinus vaninii Ljup was investigated in this study. The EPS was isolated and purified，and the molecular weight of EPS was determined by gel filtration. The type of anomeric carbon in the EPS structure was investigated by FT-IR，the carbohydrate composition of the EPS was determined by GC/MS，the molecular conformation of EPS was analyzed by SEC/MALLS binding viscosity method，the antioxidant activity of EPS was determined according to OH and DPPH radical scavenging activity. The results showed that：two EPS fractions（Fr-I and Fr-II） were obtained after separation and purification，and the relative molecular weight of Fr-I and Fr-II were 627 500 and 55 000 respectively. FTIR results showed that the Fr-I was coexistence of α- and β-configuration of mannopyranoside acidic polysaccharides，while Fr-II α-mannopyranoside acidic polysaccharides. GC/MS results showed that the monosaccharide components of Fr-I and Fr-II contained ribose，xylose，glucuronic acid，galactose，glucose，mannose and galactose；SEC/MALLS results showed that the dispersion of the EPS is very low，and the EPS kept spherical conformation in aqueous solution. The EPS is a highly compact aggregates with branch. The antioxidant activity tests showed that the ·OH scavenging rate was 38.58% when EPS concentration was 10 mg/mL，and the

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何培新,吴双双,郑凯,许春平.杨树桑黄胞外多糖的分子结构及抗氧化活性[J].食品与生物技术学报,2018,37(9):939-947.

HE Peixin, WU Shuangshuang, ZHENG Kai, XU Chunping. Molecular Structure and Antioxidant Activity of Polysaccharides from Phellinus vaninii Ljup[J]. Journal of Food Science and Biotechnology,2018,37(9):939-947.

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在线发布日期: 2018-10-30
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