新疆特色果蔬中乳酸菌分离鉴定及其抑菌活性

doi:10.3969/j.issn.1673-1689.2018.11.013

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新疆特色果蔬中乳酸菌分离鉴定及其抑菌活性
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                        10.3969/j.issn.1673-1689.2018.11.013
                    
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Identification and Antibacterial Activity of Lactic Acid Bacteria Isolated from Fruits and Vegetables Collected from Different Areas In XinJiang

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摘要:

利用MRS，M17等4种培养基对采自新疆不同地区的8种水果中的乳酸菌进行分离鉴定，根据16S rRNA基因序列确定菌株的系统进化关系，并利用牛津杯法筛选具有抑菌活性的乳酸菌。共分离疑似乳酸菌90株，rep-PCR指纹图谱分型选出15株代表菌株，经16S rRNA基因序列分析，它们分别隶属于乳杆菌属（ Lactobacillus ）8株、明串珠菌属（ Leuconostoc ）6株、乳球菌属（ Lactococcus ）1株，共3个属，8个种。利用牛津杯法筛选出8株对枯草芽孢杆菌、单增李斯特菌、金黄色葡萄球菌具有明显抑制作用的乳酸菌，为乳酸菌作为生物防腐剂应用到食品工业中奠定基础。

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The isolation and identification of lactic acid bacteria from 8 kinds of fruits collected from different regions in XinJinag were carried out by using MRS，M17 and other two different culture medium. The phylogenetic relationship was identified based on analysis of the 16s rRNA gene sequences；and the bacteriocin-producing lactic acid bacteria strains were screened by using the Oxford cup method. A total of 90 lactic acid bacteria strains were isolated from all samples，and 15 representative strains were obtained based on the rep-PCR fingerprint classification. According to the 16s rRNA gene sequence analysis，15 representative strains mainly belong to genus of Lactobacillus （8 strains）， Leuconostoc （6 strains）， Lactococcus （1 strain） respectively. A total of 8 lactic acid bacteria strains can inhibit the growth of S taphylococcus aureus ， Bacillus subtilis and Listeria monocytogenes ，were screened out by using the Oxford cup method，and they have great potential application for their possible use as a natural biopreservatives in the food industry.

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孙海龙,李佳益,张敏,曾傲琼,倪永清.新疆特色果蔬中乳酸菌分离鉴定及其抑菌活性[J].食品与生物技术学报,2018,37(11):1201-1206.

SUN Hailong, LI Jiayi, ZHANG Min, ZENG Aoqiong, NI Yongqing. Identification and Antibacterial Activity of Lactic Acid Bacteria Isolated from Fruits and Vegetables Collected from Different Areas In XinJiang[J]. Journal of Food Science and Biotechnology,2018,37(11):1201-1206.

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在线发布日期: 2018-12-25
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