麻花秦艽不同组织部位可培养内生菌群结构及其与龙胆苦苷含量的相关性

doi:10.3969/j.issn.1673-1689.2019.04.004

首页 > 过刊浏览>2019年第38卷第4期 >21-29. DOI:10.3969/j.issn.1673-1689.2019.04.004

麻花秦艽不同组织部位可培养内生菌群结构及其与龙胆苦苷含量的相关性
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                        10.3969/j.issn.1673-1689.2019.04.004
                    
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Community Structure of Cultivable Endophytes in Different Organs of Gentiana straminea Maxim and Its Correlation with the Content of Gentiopicrin

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为了明确四川麻花秦艽（Gentiana straminea Maxim）内生菌的资源状况，本实验采用稀释涂布法并结合形态学观察及16S rDNA和ITS-rDNA序列分析的方法，对其根、茎、叶和花中的内生菌进行了分离、纯化和鉴定；并采用高效液相色谱法（HPLC）检测了不同组织部位龙胆苦苷的含量，分析了麻花秦艽可培养内生菌数量与龙胆苦苷含量的相关性。结果表明，麻花秦艽不同组织部位可培养内生菌数量和菌群结构存在一定的差异。其中，从根中得到的可培养内生细菌和真菌的数量均最高，分别为（8.77±0.71）×105 cfu/g和（2.00±1.05）×104 cfu/g。统计分析显示，麻花秦艽内生细菌和内生真菌的数量分别与其龙胆苦苷的含量成极显著正相关（P<0.01）。从麻花秦艽中共分离得到13株内生菌，归属为3门6纲8目9科10属，其中细菌10株，真菌3株。其中的优势菌株，包括细菌QJ2（Duganella sp.）、QJ6（Rahnella sp.）、QJ10（Pseudomonas sp.）及真菌QJ11（Aspergillus sp.）和QJ13（Aspergillus sp.），其数量均分别与龙胆苦苷的含量成极显著正相关（P<0.01）。麻花秦艽内生菌具有较丰富的遗传多样性，不同组织部位麻花秦艽内生菌、种类及分布存在差异，并与其有效成分龙胆苦苷存在极显著正相关性。

Abstract:

In order to study the resource of the endophyte from Gentiana straminea Maxim in Sichuan，the cultivable endophyte in different organs of Gentiana straminea Maxim（roots，stems，leaves and flowers） were isolated and identified by the dilution separation methods and the sequence analysis of 16 S rDNA and ITS-rDNA. Then the content of gentiopicrin in each organ was determined by high performance liquid chromatography（HPLC） and its correlation with the abundance of cultivable endophytes was analyzed. The results showed that 13 strains of cultivable endophytes were separated from Gentiana straminea Maxim as a whole，which belong to 3 phyla，6 classes，8 orders，9 families and 10 genera，and among them，ten strains were bacteria and three were fungi. Meanwhile，there were differences of the quantity and microbial population of the cultivable endophytic bacteria and fungi in different organ or position of Gentiana straminea Maxim. The maximum quantity of endophytic bacteria and fungi were（8.77±0.71）×105 cfu/g and（2.00±1.05）×104 cfu/g respectively and both in the roots of Gentiana straminea Maxim.. Statistical analysis showed that correlations between the content of gentiopicrin and the quantity of cultivable endophytic bacteria and fungi，respectively，were extremely significantly positive（P<0.01）. More over，the quantity of each predominant strain，bacteria QJ2（Duganella sp.），bacteria QJ6（Rahnella sp.），bacteria QJ10（Pseudomonas sp.） fungi QJ11（Aspergillus sp.） and fungi QJ13（Aspergillus sp.），was extremely，significantly and positively related with the content of gentiopicrin，respectively（P<0.01）. The cultivable endophytes in Gentiana straminea Maxim were characterized by abundant genetic diversity and there were differences of the endophytes community structure in different organ of Gentiana straminea Maxim. All the results will serve as the foundation of development and utilization of endophytes in Gentiana straminea Maxim.

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陈昕,李琪,曹倩倩,黄春萍.麻花秦艽不同组织部位可培养内生菌群结构及其与龙胆苦苷含量的相关性[J].食品与生物技术学报,2019,38(4):21-29.

CHEN Xin, LI Qi, CAO Qianqian, HUANG Chunping. Community Structure of Cultivable Endophytes in Different Organs of Gentiana straminea Maxim and Its Correlation with the Content of Gentiopicrin[J]. Journal of Food Science and Biotechnology,2019,38(4):21-29.

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在线发布日期: 2019-07-03
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