产耐热β-半乳糖苷酶蜜源芽孢杆菌的鉴定及酶学性质

doi:10.3969/j.issn.1673-1689.2019.07.007

首页 > 过刊浏览>2019年第38卷第7期 >49-57. DOI:10.3969/j.issn.1673-1689.2019.07.007

产耐热β-半乳糖苷酶蜜源芽孢杆菌的鉴定及酶学性质
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                        10.3969/j.issn.1673-1689.2019.07.007
                    
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Identification of Thermostable β-Galactosidase-Producing Bacillus Strain from Honey and Enzymatic Properties

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从分离自蜂蜜的芽孢杆菌中筛选到一株高产β-半乳糖苷酶的菌株，鉴定为地衣芽孢杆菌（Bacillus licheniformis），并命名为Bacillus licheniformis SYBC hb15。纯化该酶并研究其酶学性质，以邻硝基酚-β-D-半乳糖苷（ONPG）为底物，B. licheniformis SYBC hb15所产β-半乳糖苷酶的最适水解反应温度是60 ℃；70 ℃放置60 min后酶活力仍保留65%，具有较高的热稳定性；与嗜热菌Talaromyces thermophilus来源的β-半乳糖苷酶相比，葡萄糖对其水解活性的抑制较弱。因此，B. licheniformis SYBC hb15所产的β-半乳糖苷酶具有较高的热稳定性和葡萄糖耐受性，有很好的工业应用潜力。

Abstract:

A β-galactosidase-producing strain was screened from bacillus species isolatated from honey. The strain was identified as Bacillus licheniformis by an in-depth study and named as Bacillus licheniformis SYBC hb15. β-galactosidase expressed by Bacillus licheniformis SYBC hb15 was purified for the study of enzymatic properties using o-nitrophenyl-β-D-galactoside（ONPG） as substrate. The result showed that the optimal reactive temperature of the β-galactosidase was 60 ℃，and it had high thermal stability when incubating the enzyme at 70 ℃ for 60 min with 65% residual activity. Compared to that of the enzyme from Talaromyces thermophilus，glucose presents less inhibition to the hydrolytic activity of the β-galactosidase. Therefore，the β-galactosidase produced by B. licheniformis SYBC hb15 has good potential in the industrial application because of its high thermostability and resistance to glucose of high levels.

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李云,朱少华,管政兵,蔡宇杰,廖祥儒.产耐热β-半乳糖苷酶蜜源芽孢杆菌的鉴定及酶学性质[J].食品与生物技术学报,2019,38(7):49-57.

LI Yun, ZHU Shaohua, GUAN Zhengbing, CAI Yujie, LIAO Xiangru. Identification of Thermostable β-Galactosidase-Producing Bacillus Strain from Honey and Enzymatic Properties[J]. Journal of Food Science and Biotechnology,2019,38(7):49-57.

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在线发布日期: 2020-01-23
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