豆粕蛋白在小鼠胃肠道内的吸收与分布研究

doi:10.3969/j.issn.1673-1689.2019.08.008

首页 > 过刊浏览>2019年第38卷第8期 >53-59. DOI:10.3969/j.issn.1673-1689.2019.08.008

豆粕蛋白在小鼠胃肠道内的吸收与分布研究
DOI:
                        10.3969/j.issn.1673-1689.2019.08.008
                    
作者:
                        
                        
                    
作者单位:
作者简介:
通讯作者:
中图分类号:TS201.6
基金项目:

Absorption and Distribution of Soybean Meal Protein in Mice Gastrointestinal Tract

Author:

Affiliation:

Fund Project:

摘要

图/表

访问统计

参考文献

相似文献

引证文献

资源附件

文章评论

摘要:

探讨一次性灌胃豆粕，不同时间段豆粕蛋白在小鼠胃肠道内的吸收与分布情况。以豆粕中的β-伴大豆球蛋白作为标记，采用双抗体夹心酶联免疫吸附试验技术检测豆粕在小鼠胃肠道内不同部位的吸收量，并采用免疫金银染色技术，对其主要成分豆粕蛋白在胃肠道的分布位置进行示踪，明确其吸收途径。结果表明，当小鼠摄食豆粕0.5~1 h后，豆粕蛋白主要分布于小肠内，部分于胃内，较少量残余豆粕蛋白分布于大肠内，且于摄食2~4 h，豆粕蛋白在小鼠胃肠道内的吸收趋于稳定，这与普通蛋白的吸收位置和途径是一致的。采用稳定的食物蛋白质作为标记进行示踪，为研究食物在机体胃肠道内的吸收、分布情况提供了新的思路。

Abstract:

The absorption and distribution of soybean meal protein in mice gastrointestinal tract in different time periods after single dose of soybean meal were investigated. In this study，the protein in soybean meal was used as a marker，to study the absorption of soybean meal in the gastrointestinal tract of mice. Amount of soybean meal at each part of the gastrointestinal tract was quantified by double antibody sandwich enzyme linked immunosorbent assay. The distribution of soybean protein in the gastrointestinal tract was located by immunogold silver staining. The results showed that the soybean meal protein mainly distributed in the small intestine after 0.5—1 h uptake，some in the stomach，and a few soybean meal protein was found in the large intestine，and after 2—4 h，the soybean meal protein distribution tends to be stable，which was consistent with the absorption positions and pathway of protein. Furthermore，using a stable food protein as a marker could be a new idea for studying the absorption and distribution of the food in the gastrointestinal tract.

参考文献

相似文献

引证文献

引用本文

童深广,郝红江,李洪波,陈红兵,曾辛,吴志华.豆粕蛋白在小鼠胃肠道内的吸收与分布研究[J].食品与生物技术学报,2019,38(8):53-59.

TONG Shenguang, HAO Hongjiang, LI Hongbo, CHEN Hongbing, ZENG Xin, WU Zhihua. Absorption and Distribution of Soybean Meal Protein in Mice Gastrointestinal Tract[J]. Journal of Food Science and Biotechnology,2019,38(8):53-59.

复制

文章指标

点击次数:
下载次数:
HTML阅读次数:
引用次数:

历史

收稿日期:
最后修改日期:
录用日期:
在线发布日期: 2020-03-27
出版日期:

地址：江苏省无锡市蠡湖大道1800号邮政编码：214122

电话：0510-85913526 电子邮件：xbbjb@jiangnan.edu.cn

技术支持：北京勤云科技发展有限公司

引用本文

分享

文章指标

历史

微信公众号二维码

手机版网站二维码