基于残基-水作用网络的木聚糖酶耐热性研究

doi:10.3969/j.issn.1673-1689.2019.09.011

首页 > 过刊浏览>2019年第38卷第9期 >78-84. DOI:10.3969/j.issn.1673-1689.2019.09.011

基于残基-水作用网络的木聚糖酶耐热性研究
DOI:
                        10.3969/j.issn.1673-1689.2019.09.011
                    
作者:
                        
                        
                    
作者单位:
作者简介:
通讯作者:
中图分类号:Q81
基金项目:

Research of Xylanase Thermostability Based on Residue-Water Interaction Network

Author:

Affiliation:

Fund Project:

摘要

图/表

访问统计

参考文献

相似文献

引证文献

资源附件

文章评论

摘要:

水在蛋白质折叠、相互作用、分子识别、动力学及耐热性方面发挥着重要作用。利用Voronoi算法识别出与耐热和常温木聚糖酶存在相互作用的水。以氨基酸残基和水为节点，残基-残基及残基-水相互作用为边，构建残基-水相互作用网络。计算网络中节点的度、聚类系数、接近中心性和节点中心性，发现水的这些参数均与木聚糖酶耐热性成正相关关系。耐热木聚糖酶中度为2的hub水数量要比常温酶多，可见水在耐热木聚糖酶中参与了更多的相互作用。以度为2的水为聚类中心，以氨基酸残基节点的度18为阈值进行聚类。子网表明hub水多与hub残基聚为一类，即网络存在“富人俱乐部”现象。这表明，水不仅参与了耐热木聚糖酶的残基-水相互作用，还加强了残基-残基相互作用，更有利于酶形成稳定的结构来抵御高温。

Abstract:

Water plays an important role in protein folding，protein-protein interaction，molecular recognition，protein dynamics，thermal stability and so on. In this paper，Voronoi algorithm is used to identify waters interacting with residues of xylanase. Take waters and all residues as nodes，and take residue-residue interactions and residue-water interactions as edges，we constructed residue-water interaction networks. After calculating network topology parameters such as the network node degree，clustering coefficient，closeness centrality and node centrality，we found that these parameters were positively correlated with the thermal stability of xylanase. The number of hub water with degree 2 in thermophilic xylanase network is much higher than in mesophilic xylanase network，which means water is involved in more interaction in thermostable xylanase. Then take hub water with degree 2 as clustering center and set residue degree≥18 as threshold degree，we modularized the networks. Analyzing the sub-networks obtained from clustering the whole network，we found hub waters and hub residues are usually clustered into a group. That means residue-water interaction networks of xylanase exist "rich-club phenomenon". These results indicate that water is not only involved in the residue-water interaction，but also strengthen the residue-residue interaction，which is more helpful to the formation of a stable structure to resist high temperature.

参考文献

相似文献

引证文献

引用本文

饶榕,丁彦蕊.基于残基-水作用网络的木聚糖酶耐热性研究[J].食品与生物技术学报,2019,38(9):78-84.

RAO Rong, DING Yanrui. Research of Xylanase Thermostability Based on Residue-Water Interaction Network[J]. Journal of Food Science and Biotechnology,2019,38(9):78-84.

复制

文章指标

点击次数:
下载次数:
HTML阅读次数:
引用次数:

历史

收稿日期:
最后修改日期:
录用日期:
在线发布日期: 2020-03-31
出版日期:

地址：江苏省无锡市蠡湖大道1800号邮政编码：214122

电话：0510-85913526 电子邮件：xbbjb@jiangnan.edu.cn

技术支持：北京勤云科技发展有限公司

引用本文

分享

文章指标

历史

微信公众号二维码

手机版网站二维码