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文章摘要
铁钉菜水提醇沉上清液部分的抗氧化、抗酪氨酸酶活性及其冲剂的降糖作用
Antioxidant and Anti-Tyrosinase Activities of Supernatants from Water Extracting-Alcohol Precipitation of Ishige okamurai and Hypoglycemic Effect of Its Granules
  
DOI:10.3969/j.issn.1673-1689.2019.11.020
中文关键词: 铁钉菜  水提醇沉上清液部分  抗氧化  抗酪氨酸酶  冲剂  降血糖
英文关键词: Ishige okamurai Yendo,supernatants from water extracting-alcohol precipitation,antioxidant activity,anti-tyrosinase,granules,hypoglycemic
基金项目:
作者单位
黄晓冬 泉州师范学院 海洋与食品学院福建 泉州 362000
华侨大学 福建省分子医学重点实验室福建 厦门 361021 
黄世英 泉州师范学院 海洋与食品学院福建 泉州 362000
福建省海洋藻类活性物质制备与功能开发重点实验室福建 泉州 362000 
蔡建秀 泉州师范学院 海洋与食品学院福建 泉州 362000 
王玲平 泉州师范学院 海洋与食品学院福建 泉州 362000 
孙波 泉州师范学院 海洋与食品学院福建 泉州 362000 
谢秋燕 泉州师范学院 海洋与食品学院福建 泉州 362000 
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中文摘要:
      为探讨海藻铁钉菜提取物的抗氧化、抗酪氨酸酶活性与降糖作用,作者采用DPPH、羟基自由基清除体系测定铁钉菜水提醇沉上清液部分(SWEAPI)的抗氧化活性,采用多巴色素法测定其抗酪氨酸酶活性,并以可溶性淀粉、甜蜜素、β-环状糊精为辅料,采用正交实验设计的优化条件制备铁钉菜固体冲剂;通过体外α-葡萄糖苷酶活性抑制实验与体内四氧嘧啶致糖尿病小鼠模型,研究该冲剂的降血糖作用。结果表明:SWEAPI具有较强的自由基清除能力与抗酪氨酸酶活性,其DPPH自由基清除的半效应质量浓度EC 50 为1.19 mg/mL,羟基自由基清除的EC 50 为5.08 mg/mL,酪氨酸酶活性抑制的半效应质量浓度IC 50 为1.27 mg/mL;这可能与其较高的多酚含量(8.30%)有关。采用SWEAPI:可溶性淀粉:甜蜜素:β-环状糊精为30∶60∶1∶7.5的配比进行铁钉菜冲剂制备可获得较好的产品质量,冲剂的水分质量分数≤10%、总糖质量分数约为18.74 %、蛋白质质量分数16.60%、多酚质量分数2.53%。该冲剂具有较强的α-葡萄糖苷酶抑制活性,半效应质量浓度IC50为0.37 mg/mL。口服冲剂14 d后,与模型组比较,350 mg/kg的使用剂量时可极显着降低糖尿病小鼠血糖,糖耐量实验进一步证实其可以在短时间内降低餐后血糖,总体上,其降糖效果与阳性药物二甲双胍处理组(500 mg/kg)相近。可见,SWEAPI具有较强的抗氧化活性、抗酪氨酸酶活性与降糖作用,具有开发成海藻源护肤品、降糖功能产品的潜在应用价值。
英文摘要:
      The study aimed to investigate the antioxidant,anti-tyrosinase,and hypoglycemic activities of the extracts from Ishige okamurai.The antioxidant activities of the supernatants from water extracting-alcohol precipitation of Ishige okamurai(hereafter,referred to as simply SWEAPI) were determined by the DPPH and hydroxyl radical scavenging system,its anti-tyrosinase activity was determined by the dopachrome method. Using SWEAPI as basic material and soluble starch,sodium cyclamate,and β-cyclodextrine as auxiliary materials,the granules were prepared by the optimization conditions from orthogonal experiment. The hypoglycemic effects of granules were studied by in vitro α-glycosidase enzyme activity inhibition experiment and in vivo alloxan-induced diabetic mice. The results show that EC50 of DPPH scavenging is 1.19 mg/mL,EC50 of hydroxyl radical scavenging is 5.08 mg/mL,and IC50 of tyrosinase inhibition is 1.27 mg/mL. These phenomena are related to its higher polyphenol content(8.30%). When the granules are made by the optimization conditions with the 30∶60∶1∶7.5 ratio of SWEAPI:soluble starch:sodium cyclamate:β-cyclodextrine,it has the best product quality with content of water(≤10%),total sugar(18.74 %),protein(16.60%),and polyphenol(2.53%). The granules have strong inhibitory activity against α-glycosidase enzyme with IC50 of 0.37 mg/mL. Compared with the model control group,the granules could significantly reduce the blood glucose level of diabetes mice after 14 days of treatment with oral dose of 350 mg/kg. The granules also could reduce postprandial blood glucose level in a short time by the results of glucose tolerance experiment,which are similar to positive drug metformin at dose of 500 mg/kg. In conclusion,SWEAPI has strong antioxidant,anti-tyrosinase,and hypoglycemic activities with potential applications in skin care products and hypoglycemic functional products.
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