酿酒酵母萌发突变株的筛选

doi:10.3969/j.issn.1673-1689.2019.12.013

首页 > 过刊浏览>2019年第38卷第12期 >91-96. DOI:10.3969/j.issn.1673-1689.2019.12.013

酿酒酵母萌发突变株的筛选
DOI:
                        10.3969/j.issn.1673-1689.2019.12.013
                    
作者:
                        
                        
                    
作者单位:
作者简介:
通讯作者:
中图分类号:Q93
基金项目:

Isolation of Germination Mutants in Saccharomyces cerevisiae

Author:

Affiliation:

Fund Project:

摘要

图/表

访问统计

参考文献

相似文献

引证文献

资源附件

文章评论

摘要:

酵母细胞孢子作为微胶囊首次运用于酶的固定化，但是由于其孢子遇到营养物质即萌发的性质，导致固定化酶的重复利用率受到限制，因此筛选有萌发缺陷的酵母细胞孢子突变株对微胶囊的应用至关重要。利用EMS对酵母细胞进行诱变再经过乙醚的筛选获得4株萌发突变株AN120-m5、AN120-m8、AN120-m10和AN120-m17。验证后发现他们均产孢正常，除了AN120-m5生长有些弱之外，其余3株生长都正常。后对这些突变株进行了液体培养基以及固体培养基内的验证，实验发现所有突变株都表现出明显的萌发缺陷，其中AN120-m10孢子在液体培养基中萌发率只有1%，而AN120-m5单个孢子在固体培养基上则完全不萌发。由于AN120-m5单个孢子不能萌发因此得不到杂交后代，而其余3株突变株在与野生型杂交三代后，发现野生型孢子与突变型孢子萌发状况相比始终呈现2∶2的萌发优劣比，从而可以推测突变株是单基因突变菌株，为后续研究确定突变基因从而筛选得到完全不萌发菌株打下基础。

Abstract:

The spores of Saccharomyces cerevisiae firstly were applied for enzyme immobilization as microcapsule. The utilization of the enzyme has been limited due to germination of spores when treated with nutrients，thus finding germination mutant yeast spore has a significant effect on applying of microcapsule. We used EMS as a mutagen for yeast cells and screened by ether and isolated 4 mutants，AN120-m5，AN120-m8，AN120-m10 and AN120-m17. They all can sporulate and grew well except that AN120-m5 was slightly deficient in cell growth. When verifying the germination efficiency of 4 mutants in the liquid medium and on solid medium，4 mutants all exhibited obvious germination defect，however only 1% AN120-m10 spores germinated in the liquid medium，and single spore of AN120-m5 completely cannot germinate on solid medium. Hybrid cells of AN120-m5 cannot be collected because of its defect on solid medium，and other 3 mutant haploid cells crossed with wide type haploid cells after 3 generations，and spores of wild type exhibited a germination eudismic ratio of 2∶2 compared with mutant spores. This suggested that a single gene was mutated in mutant strains will facilitate to find mutated gene in later screening in order to isolate a strain that completely doesn’t germinate.

参考文献

相似文献

引证文献

引用本文

邱盼盼,高晓冬,中西秀树.酿酒酵母萌发突变株的筛选[J].食品与生物技术学报,2019,38(12):91-96.

QIU Panpan, GAO Xiaodong, NAKANISHI Hideki. Isolation of Germination Mutants in Saccharomyces cerevisiae[J]. Journal of Food Science and Biotechnology,2019,38(12):91-96.

复制

文章指标

点击次数:
下载次数:
HTML阅读次数:
引用次数:

历史

收稿日期:
最后修改日期:
录用日期:
在线发布日期: 2020-04-07
出版日期:

地址：江苏省无锡市蠡湖大道1800号邮政编码：214122

电话：0510-85913526 电子邮件：xbbjb@jiangnan.edu.cn

技术支持：北京勤云科技发展有限公司

引用本文

分享

文章指标

历史

微信公众号二维码

手机版网站二维码