外源氧载体和前体L-谷氨酸添加策略提高Bacillus subtilis HB-1发酵产γ-聚谷氨酸

doi:10.3969/j.issn.1673-1689.2020.06.012

首页 > 过刊浏览>2020年第39卷第6期 >84-92. DOI:10.3969/j.issn.1673-1689.2020.06.012

外源氧载体和前体L-谷氨酸添加策略提高Bacillus subtilis HB-1发酵产γ-聚谷氨酸
DOI:
                        10.3969/j.issn.1673-1689.2020.06.012
                    
作者:
                        
                        
                    
作者单位:
作者简介:
通讯作者:
中图分类号:Q815
基金项目:

Addition of Exogenous Oxygen Carrier and Precursor L-Glutamic Acid Enhanced the Production of γ-Poly Glutamic Acid by Fermentation of Bacillus subtilis HB-1

Author:

Affiliation:

Fund Project:

摘要

图/表

访问统计

参考文献

相似文献

引证文献

资源附件

文章评论

摘要:

为了提高外源L-谷氨酸依赖性菌株枯草芽孢杆菌HB-1产γ-聚谷氨酸（γ-PGA）的能力，采用添加氧载体和前体L-谷氨酸的策略，研究其对菌体生长和γ-PGA产量的影响。摇瓶单因素结果表明，最佳氧载体为聚二甲氧基硅烷（PDMS），在原始培养基中添加体积分数为10%的PDMS，菌体OD600提高了3～5倍；前体L-谷氨酸的最适添加时间为18 h，最适添加质量浓度为10 g/L。在3 L发酵罐扩大培养后，γ-PGA产量提高到45 g/L；在发酵30 h流加碳源和前体L-谷氨酸，γ-PGA质量浓度达到52 g/L。发酵产物经红外，氨基酸分析仪等证实其为多肽类化合物，相对分子质量高达1千万。

Abstract:

In order to improve the ability of exogenous L-glutamate-dependent strain Bacillus subtilis HB-1 to produce γ-poly glutamic acid (γ-PGA), the study explored the effects of adding oxygen carrier and precursor L-glutamic acid on the cell growth and the yield of γ-PGA. Based on single factor tests, the optimum oxygen carrier was polydimethoxysilane (PDMS), and the cell density increased 3～5 times after the addition of 10% PDMS (v/v) in the initial fermentation medium; moreover, the optimum addition time of precursor L-glutamic acid was 18 h, and the optimum concentration was 10 g/L. With the optimal conditions, the concentration of γ-PGA increased to 45 g/L in a 3 L fermenter, and its yield reached 52 g/L by the combination of feeding carbon source and L-glutamic acid in 30 h. The fermentation product was confirmed to be a polypeptide compound by infrared and amino acid analyzers, and the molecular weight of γ-PGA was up to 10 million dalton.

参考文献

相似文献

引证文献

引用本文

任东雪,陈鹏程,郑璞,徐志南,卢松.外源氧载体和前体L-谷氨酸添加策略提高Bacillus subtilis HB-1发酵产γ-聚谷氨酸[J].食品与生物技术学报,2020,39(6):84-92.

REN Dongxue, CHEN Pengcheng, ZHENG Pu, XU Zhinan, LU Song. Addition of Exogenous Oxygen Carrier and Precursor L-Glutamic Acid Enhanced the Production of γ-Poly Glutamic Acid by Fermentation of Bacillus subtilis HB-1[J]. Journal of Food Science and Biotechnology,2020,39(6):84-92.

复制

文章指标

点击次数:
下载次数:
HTML阅读次数:
引用次数:

历史

收稿日期:
最后修改日期:
录用日期:
在线发布日期: 2020-10-20
出版日期:

地址：江苏省无锡市蠡湖大道1800号邮政编码：214122

电话：0510-85913526 电子邮件：xbbjb@jiangnan.edu.cn

技术支持：北京勤云科技发展有限公司

引用本文

分享

文章指标

历史

微信公众号二维码

手机版网站二维码