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文章摘要
水相体系中Amadori化合物的合成及检测方法研究
Synthesis and Detection Methods of Amadori Compounds in Aqueous System
  
DOI:10.3969/j.issn.1673-1689.2020.07.009
中文关键词: 美拉德反应  Amadori化合物  超高效液相色谱-串联四级杆质谱(UPLC-MS/MS)  核磁共振(NMR)
英文关键词: Maillard reaction, Amadori compound, ultra high performance liquid chromatography- tandem mass spectrometry(UPLC-MS/MS), nuclear magnetic resonance(NMR)
基金项目:
作者单位
李硕 江南大学 食品学院江苏 无锡 214122 
张建 石河子大学 食品学院新疆 石河子 832003 
张连富 江南大学 食品学院江苏 无锡 214122
石河子大学 食品学院新疆 石河子 832003
北京工商大学 北京食品营养与人类健康高精尖创新中心北京 100048 
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中文摘要:
      Amadori化合物(Amadori Compounds,ACs)是美拉德反应早期形成的稳定产物,普遍存在于热加工处理的蔬菜和水果等制品中,对产品品质有重要影响。在已经完成的8种ACs合成研究基础上,建立水相体系中合成1-脱氧-1-L-脯氨酸-D-果糖(Fru-Pro)、1-脱氧-1-L-丝氨酸-D-果糖(Fru-Ser)、1-脱氧-1-L-苏氨酸-D-果糖(Fru-Thr)和1-脱氧-1-L-甘氨酸-D-果糖(Fru-Gly) 4种ACs组分,经超高效液相色谱-串联四级杆质谱(UPLC-MS/MS)、核磁共振(NMR)等手段表征后,确定其分别为Fru-Pro(C11H19NO7,MW:277),Fru-Ser(C9H17NO8,MW:267),Fru-Thr(C10H19NO8,MW:281),Fru-Gly(C8H15NO7,MW:237)。采用液质联用仪建立了同步测定全部12种ACs的方法,并对该方法进行了方法学验证,结果表明12种ACs在检测范围内线性关系良好,相关系数均大于0.99,对目标物的分离效果好,灵敏度高。最后对市场上13种果蔬干进行ACs含量的检测,结果显示该方法检测效果好,可以检测出产品中所含的ACs。
英文摘要:
      Amadori compounds (AC) are stable products formed in the early stage of Maillard reaction. They are commonly found in heat processed vegetables, fruits and other food products and have an important impact on food quality. In the previous study, we synthesized 8 AC and this paper aimed to establish the synthesis of 1-deoxy-1-L-proline-D-fructose(Fru-Pro),1-deoxy-1-L-serine(Fru-Ser),1-deoxy-1-L-threonine-D-fructose(Fru-Thr), and 1-deoxy-1-L-glycine-D-fructose (Fru-Gly) in the aqueous systems. Four purified AC were determined to be Fru-Pro (C11H19NO7, MW: 277), Fru-Ser (C9/H17NO8, MW: 267), Fru-Thr (C10H19NO8, MW: 281) and Fru-Gly (C8H15NO7, MW: 237), respectively, by ultra high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) and nuclear magnetic resonance(NMR). In addition, an UPLC-MS/MS method was developed for the simultaneous determination of 12 AC. The results showed that the recovery, precision, and linearity data were satisfactory. Finally, the AC contents of 13 dried fruits and vegetables on the market were tested. The results showed that the method had good detection capacitiy and could detect AC contained in the products.
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