Abstract:This study aimed to investigate the alterations in the gut microbiota of mice sensitized with ovalbumin (OVA) without immunologic adjuvant treatment, and to explore the characteristic gut microbiota associated with OVA allergy. An OVA-sensitized mouse model was constructed, and the allergic reactions of the mice were evaluated by the allergic symptom score, detection of serum immunoglobulin E(IgE) level, and observation of colon histological morphology. Gut microbiota analysis was performed using 16S rRNA amplicon sequencing, including diversity analysis, species composition analysis, and functional prediction. The results showed that there were significant changes in the allergic symptom score, total serum IgE, OVA-specific IgE, and HE staining results of colon tissue in the OVA-sensitized group, indicating the success of model construction. The gut microbiota analysis revealed a significant increase in the abundance of Pseudomonadaceae at the family level, a significant decrease in the abundance of Lachnospirales-Lachnospiraceae and Desulfovibrionales-Desulfovibrionaceae, and a significant increase in the abundance of Pseudomonas at the genus level, together with a significant decrease in the abundance of Lachnospiraceae NK4A136 group in the sensitized group. Bacteroidales could be speculated as a characteristic microbiota of OVA allergy.
