基于HS-SPME-GC-MS对广西12种红茶香气成分分析
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广西南亚热带农业科学研究所

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O657.63; TS272.7

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广西科技基地和人才专项(桂科AD23026328),广西农业科技创新联盟项目(桂农科盟202406-1),广西农科院科技发展基金项目(桂农科2023YM127)


Analysis of Aroma Components of 12 Black Teas From Guangxi Province Based on HS-SPME-GC-MS
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Guangxi South Subtropical Agricultural Sciences Research Institute

Fund Project:

Guangxi Science and Technology Base and Talent Special Project (Guike AD23026328), Guangxi Agricultural Science and Technology Innovation Alliance Project (Guikemeng 202406-1), Science and Technology Development Project of Guangxi Academy of Agricultural Sciences (Guinongke 2023YM127)

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    摘要:

    为了分析广西不同茶树品种红茶的香气挥发性组分特征及其关键呈香物质,本文采用感官审评结合顶空固相微萃取技术结合气质联用仪分析鉴别来自广西12个红茶样品香气成分,通过聚类分析和正交偏最小二乘判别分析(OPLS-DA)。结果表明,不同品种红茶香气品质不同,主要呈现花、果香型和甜香型香气特征,乌牛早红茶(91.20%)综合评分最高,是甜香型红茶代表,黄观音红茶(90.55)综合评分次之,是花、果香型红茶代表。12个品种红茶共检测出醇类、醛类、酮类、酯类、烯烃类、吡咯类及其衍生物和其他类40种香气化合物,其中醇类、酯类和醛类是主要的香气贡献物质,相对含量分别是59.84%-79.21%、4.25%-17.68%和2.93%-13.65%。基于33种共有香气物质建立OPLS-DA模型,能明显区分花、果香型和甜香型红茶,并且鉴别出脱氢芳樟醇、芳樟醇氧化物Ⅱ、芳樟醇氧化物Ⅰ、香叶醇、水杨酸甲酯、(E)-2-己烯醛、芳樟醇、β-紫罗酮是花、果香型和甜香型两类红茶的关键差异化合物。

    Abstract:

    In order to analyze the characteristics of aroma volatile components and key aroma components of black tea from different tea tree varieties in Guangxi, The 12 black tea samples of different varieties in Guangxi province, were identified with sensory quality evaluation combined with headspace solid-phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry (GC-MS), and the samples were analyzed by hierarchical cluster analysis and orthogonal partial least squares discrimination analysis (OPLS-DA). The results showed that different varieties of black tea aroma quality were different, the black tea mainly presented floral fruity, and sweet aromas, The sensory quality of Wu Niuzao black tea was the best, followed by Huang Guanyin, the total score was 91.20, 90.55, respectively, and the Wu Niuzao was representative of sweet black tea, Huang Guanyin was representative of floral, fruity black tea. A total of 40 aroma compounds were detected including alcohols, aldehydes, ketones, esters, olefins, pyrroles and their derivatives, and others. Among them, alcohols, esters, and aldehydes were the main aroma contributors substances, and their relative content was 59.84%-79.21%, 3.80%-17.68%, and 2.93%-16.65%, respectively. OPLS-DA models, which enabled the effective distinguishing of sweet-odor black tea and floral fruity-odor black tea, were constructed based on the relative contents of 33 common aroma compounds. Dehydrolinalool, linalool oxidized II, linalool oxide Ⅰ, geraniol, methyl salicylate, (E)-2-hexenal, linalool, and β-ionone, and were identified as the key differentiating compounds between the two types of black tea of floral fruity-odor and sweet-odor.

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  • 收稿日期:2024-03-10
  • 最后修改日期:2024-04-07
  • 录用日期:2024-04-08
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