载咖啡酸苯乙酯纳米颗粒构建及抗氧化性研究
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1.上海理工大学 健康科学与工程学院;2.上海健康医学院 药学院;3.上海理工大学 健康科学与工程学院

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国家自然科学基金项目(面上项目,重点项目,重大项目)


Fabrication of caffeic acid phenethyl ester-loaded nanoparticles and study of their antioxidant properties
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1.School of Health Sciences and Engineering,University of Shanghai for Science and Technology;2.School of Pharmacy, Shanghai University of Medicine and Health Sciences

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    摘要:

    为改善CAPE在水溶液中的分散度和利用度,本研究以反溶剂分散法构建了载CAPE大麦醇溶蛋白纳米颗粒(CAPE-HD),对其理化性能进行了表征,并对其在Caco-2细胞内的抗氧化性能进行了考察。结果显示,CAPE-HD纳米颗粒中各组分间通过氢键等非共价作用相互结合,并在形成纳米颗粒后,CAPE的存在状态发生了改变。CAPE-HD纳米颗粒呈现近球形颗粒,平均粒径为193.93 nm,平均PDI为0.26,平均Zata电位为14.50 mV,并在28天内保持稳定。纳米颗粒具有良好的包封率和载药率,以及优越的生物相容性。抗氧化实验显示CAPE-HD纳米颗粒无论在DPPH自由基的清除方面,还是在Caco-2细胞内,都表现出良好的抗氧化活性。该纳米体系为提高CAPE等难溶性药物的生物利用度和临床使用率提供了一个新的平台和思路。

    Abstract:

    In order to improve the dispersion and utilization of CAPE in aqueous solution, CAPE-loaded barley alcohol soluble protein nanoparticles (CAPE-HD) were fabrication by the anti-solvent dispersion method in this study, and their physicochemical properties were characterized and their antioxidant properties in Caco-2 cells were investigated. The results showed that the components in CAPE-HD nanoparticles bonded with each other through non-covalent operations such as hydrogen bonding, and the state of existence of CAPE changed after the formation of the nanoparticles. CAPE-HD nanoparticles showed near-spherical particles, with an average size of 193.93 nm, an average PDI of 0.26, and an average Zata potential of 14.50 mV, and remained stable for 28 days. The nanoparticles had good encapsulation and drug loading rates with excellent biocompatibility. Antioxidant experiments showed that CAPE-HD nanoparticles exhibited good antioxidant activity both in terms of DPPH radical scavenging and in Caco-2 cells. The nanosystem provides a new platform and idea to improve the bioavailability and clinical use of insoluble drugs such as CAPE.

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  • 收稿日期:2024-03-29
  • 最后修改日期:2024-04-18
  • 录用日期:2024-04-19
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