Enzyme Linked Immunosorbent Assay for the Detection of Sudan Ⅰ

doi:10.3969/j.issn.1673-1689.2009.06.014

Home > Archive>Volume 28, Issue 6, 2009 >791-794. DOI:10.3969/j.issn.1673-1689.2009.06.014

Enzyme Linked Immunosorbent Assay for the Detection of Sudan Ⅰ
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                        10.3969/j.issn.1673-1689.2009.06.014
                    
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A fast and reliable SudanI determine method with ELISA and anti-SudanI McAb 12D8 was developed in this study.It was found that the determination limitation was 4.22 ng/mL and the linear range of the inhibition curve was 7.8-125 ng/mL,respectively.In the detection of the samples of mixed chilli powder with SudanI(80~4000 ng/g),extracted with methanol:water(70:30,v/v),the recovery rate of Sudan Ⅰ in the samples ranged from 52.3% to 110%,and the coefficients of variation was 3.2% to 8.9%.

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TU Shun, QIU Xue-mei, XIE Shao-xia, WU Qian-hui, LIU Ren-rong. Enzyme Linked Immunosorbent Assay for the Detection of Sudan Ⅰ[J]. Journal of Food Science and Biotechnology,2009,28(6):791-794.

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