Characteristics and Performances of National Agrose Cation Exchanger and Its Application in Lysozyme Purification from Egg White

doi:10.3969/j.issn.1673-1689.2009.06.016

Home > Archive>Volume 28, Issue 6, 2009 >799-803. DOI:10.3969/j.issn.1673-1689.2009.06.016

Characteristics and Performances of National Agrose Cation Exchanger and Its Application in Lysozyme Purification from Egg White
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                        10.3969/j.issn.1673-1689.2009.06.016
                    
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The national cation exchanger(Zhengguang CM-Agrose Fast Flow) was integrated evaluated on its physic properties,functional group density,static and dynamic adsorption properties.Besides,the adsorbents were used to purification of lysozyme from egg white.As a result,it was found that the physic properties of CM-Agrose FF include density,water content and size distribution are up to the requirement.The functional group density reaches 187 mmol/mL,which is higher than CM Sepharose FF.The static and dynamic adsorption of lysozyme reach 107.0 and 90.3 mg/mL(100 cm/h),respectively,which indicated that the adsorbents could fulfil the efficient adsorption of proteins.Lysozyme was successfully purified from egg white using the cation exchanger.The purity of lysozyme was more than 95% and the yield reached 4.1 mg/mL egg white.

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XIA Hai-feng, JIN Xiong-hua, RAO Zhi-ming, HAO Fei, YAN Wei, ZHENG Zhi-yong. Characteristics and Performances of National Agrose Cation Exchanger and Its Application in Lysozyme Purification from Egg White[J]. Journal of Food Science and Biotechnology,2009,28(6):799-803.

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