Cloning and Prokaryotic Expression Vector Construction of Bovine Chymosin

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Cloning and Prokaryotic Expression Vector Construction of Bovine Chymosin
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In this study,the total RNA of chymosin was extracted from Absomum of calf and its cDNA was obtained by RT-PCR.The purified RT-PCR products and pMD-18T vector were ligated and transformed into host strain E.coli JM109.The full length of Chymosin gene is consist of 1 143 bp,and encodes 381 amino acids.The homology of the nucleotides with NM180994 is 99.56%.The aim gene was expression on the prokaryotic expression vector pET-22b and a recombinant plasmid pET-22b/Chymosin was constructed successfully.

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ZHANG Li, JIANG Yuan-yuan, ZHANG Jian, YANG Zhen-nai. Cloning and Prokaryotic Expression Vector Construction of Bovine Chymosin[J]. Journal of Food Science and Biotechnology,2010,29(2):271-275.

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Online: June 17,2014
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