Determination of Saponins from the Mycelia of Termitomyces albuminosus in Submerged Culture

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Determination of Saponins from the Mycelia of Termitomyces albuminosus in Submerged Culture
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In this study,the spectrophotometric method was used for determining the content of saponins from the mycelia in submerged culture.Ginsenoside Rg1 as the standard,at 550 nm,the linear range of the saponins concentration was from 10 μg to 240 μg,and the regressive equation was Y= 0.003 0 X-0.040 5,R2=0.998 4.The recovery rate was 99.4% and the relative standard deviation(RSD) was 1.97%.The relative standard deviation(RSD) of the accuracy was 1.89%.The content of saponins from the mycelia in submerged culture was about 2.69 mg/g.The method presented here has been proven to be convenient to operate and the result is accurate and highly sensitive.

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NIE Xiao-dong, XU Hong-yu, LU Zhen-ming, XU Zheng-hong. Determination of Saponins from the Mycelia of Termitomyces albuminosus in Submerged Culture[J]. Journal of Food Science and Biotechnology,2010,29(2):288-293.

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