Cloning of β-Galactosidase Gene from Lactobacillus acidophilus and Study of Its Activity as a Food-Grade Selection Marker

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Cloning of β-Galactosidase Gene from Lactobacillus acidophilus and Study of Its Activity as a Food-Grade Selection Marker
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To study the selective activity and stability of β-galactosidase gene(lacZ) from Lactobacillus acidophilus as a food-grade selection marker,the lacZ gene from L.acidophilus was cloned and expressed in L.lactis.In this study,the selective activity was determined by X-gal-containing medium and the activity and specific activity of β-galactosidase by X-gal.The results showed that the positive recombinant strains were selected successfully by β-galactosidase activity and X-gal coloration.The β-galactosidase specific activity of the recombinant L.lactis after a period of 60 generations selected with X-gal was similar to that of the 2nd generation(P=0.592>0.05),and also similar to the 61st generation selected with Erythromycin(P=0.882>0.05).These results indicated that the β-galactosidase lacZ gene had good activity and stability as a selection marker,and it could be used as a selection marker for construction of a food-grade vector.

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HE Song, GONG Fang-hong, ZHANG De-chun, LIU Ming-fang, CHENG Fang, GUO Ya-nan. Cloning of β-Galactosidase Gene from Lactobacillus acidophilus and Study of Its Activity as a Food-Grade Selection Marker[J]. Journal of Food Science and Biotechnology,2010,29(5):777-781.

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Online: June 17,2014
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