Resonance Light Scattering Detection of Adenosine Based on Aptamer Functionalized Nanogold Probe Recognition

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Resonance Light Scattering Detection of Adenosine Based on Aptamer Functionalized Nanogold Probe Recognition
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Abstract:

Coupling nano-probe technology,aptamer recognition with resonance light scattering analysis,through recognizing reaction between adenosine and its specific aptamer in homogeneous matrix,causing the disaggregation of the aggregated network structure of adenosine specific aptamer functionalized nanogold structure,a sharply change on the resonance light scattering signal can be obtained.Based on it,a novel homogeneous aptamer recognizing based method for adenosine detection was established.The linear concentration range was 0.1～10μmol/L for adenosine with a detection limit of 0.04μmol/L(3S/N).The proposed method also shows good selectivity for adenosine detection and no interference from guanosine,thymidine,cytidine and uridine.

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WANG Zhou-ping, DUAN Nuo, PENG Xiao-li, LE Guo-wei. Resonance Light Scattering Detection of Adenosine Based on Aptamer Functionalized Nanogold Probe Recognition[J]. Journal of Food Science and Biotechnology,2010,29(6):889-894.

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Online: June 17,2014
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