Optimization of Trichoderma konigii Cellulase Production on Solid-State Fermentation Medium by Response Surface Analysis

doi:10.3969/j.issn.1673-1689.2011.01.019

Home > Archive>Volume 30, Issue 1, 2011 >106-112. DOI:10.3969/j.issn.1673-1689.2011.01.019

Optimization of Trichoderma konigii Cellulase Production on Solid-State Fermentation Medium by Response Surface Analysis
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                        10.3969/j.issn.1673-1689.2011.01.019
                    
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Corn bran was used as the substrate for cellulase production using Trichoderma konigii to achieve its high value utilization,and the nutritional and envionmental conditions were optimized for enhancing cellulase yield.Such key parameters as wheat bran addition,initial moisture content and pH were optimized by Box Behnken experimental design.Experimental results showed that the impact order of three key parameters on cellulase production ware listed as follows: initial moisture content > wheat bran addition > pH,while the dependence between three factors was not significant.Predicted results indicated that cellulase production was high up to 11.3 FPAase units / g dry substrate after the fermentation of 5 days with 24.4% of wheat bran addition,1.0% of ammonium sulfate,0.05% of potassium dihydrogen phosphate,0.1% of magnesium sulfate,0.2% of lactose,58.6% of the initial water content and pH5.5.

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CHEN Xiao-ping, SUN Fu-bao, CHEN Xiao-xu, ZHANG Jian-hua, ZHANG Hong-jian, MAO Zhong-gui. Optimization of Trichoderma konigii Cellulase Production on Solid-State Fermentation Medium by Response Surface Analysis[J]. Journal of Food Science and Biotechnology,2011,30(1):106-112.

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Online: June 17,2014
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